High-throughput platform for real-time monitoring of biological processes by multicolor single-molecule fluorescence

机译：高通量平台可通过多色单分子荧光实时监测生物过程

代理获取

本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。

页面导航

摘要
著录项
相似文献
相关主题

摘要

Zero-mode waveguides provide a powerful technology for studying single-molecule real-time dynamics of biological systems at physiological ligand concentrations. We customized a commercial zero-mode waveguide-based DNA sequencer for use as a versatile instrument for single-molecule fluorescence detection and showed that the system provides long fluorophore lifetimes with good signal to noise and low spectral cross-talk. We then used a ribosomal translation assay to show real-time fluidic delivery during data acquisition, showing it is possible to follow the conformation and composition of thousands of single biomolecules simultaneously through four spectral channels. This instrument allows high-throughput multiplexed dynamics of single-molecule biological processes over long timescales. The instrumentation presented here has broad applications to single-molecule studies of biological systems and is easily accessible to the biophysical community.

机译：零模波导为研究生理系统配体浓度下的生物系统的单分子实时动力学提供了强大的技术。我们定制了一种基于商业零模波导的DNA测序仪，用作单分子荧光检测的多功能仪器，并表明该系统具有较长的荧光团寿命，并具有良好的信噪比和低光谱串扰。然后，我们使用核糖体翻译测定法来显示数据采集过程中的实时流体递送，这表明可以通过四个光谱通道同时跟踪数千个生物分子的构象和组成。该仪器可在很长的时间范围内实现单分子生物过程的高通量多重动力学。这里介绍的仪器在生物系统的单分子研究中具有广泛的应用，并且生物物理界很容易获得。

著录项

期刊名称 Proceedings of the National Academy of Sciences of the United States of America
作者
Jin Chen; Ravindra V. Dalal; Alexey N. Petrov; Albert Tsai; Seán E. O’Leary; Karen Chapin; Janice Cheng; Mark Ewan; Pei-Lin Hsiung; Paul Lundquist; Stephen W. Turner; David R. Hsu; Joseph D. Puglisi;
展开▼
作者单位

展开▼
年(卷),期 2014(111),2
年度 2014
页码 664–669
总页数 6
原文格式 PDF
正文语种
中图分类
关键词

相似文献

外文文献
中文文献
专利

1. Use of a Real-Time Fluorescence Monitoring System for High-Throughput Screening for Prolyl Isomerase Inhibitors [J] . Mori T, Itami S, Yanagi T, Journal of biomolecular screening: The official journal of the Society for Biomolecular Screening . 2009,第4期

机译：高通量筛选脯氨酰异构酶抑制剂的实时荧光监测系统的使用
2. Residual Copper(II) Detection in Chemical Processes: High-Throughput Analysis and Real-Time Monitoring with a Colorimetric Copper Probe [J] . Jung Jiyoung, Jo Junyong Organic process research & development . 2019,第6期

机译：化学过程中的残留铜（II）检测：高通量分析和使用比色铜探头的实时监测
3. Six-in-one peptide functionalized upconversion@polydopamine nanoparticle-based ratiometric fluorescence sensing platform for real-time evaluating anticancer efficacy through monitoring caspase-3 activity [J] . Lin Liu, Xiaotong Li, Hua Zhang, Sensors and Actuators . 2021,第Apra期

机译：六合一肽官能化上转化@聚二胺纳米粒子基比例荧光传感平台，用于通过监测Caspase-3活性实时评估抗癌效果
4. High-throughput and real-time microalgae monitoring platform using lens-free shadow imaging system (LSIS) [C] . Dongmin Seo, M. Roy, Jaewoo Kim, IEEE Sensors . 2016

机译：使用透镜阴影成像系统（LSIS）的高通量和实时微藻监测平台
5. Implementation of Low Cost, High-Throughput and High Sensitive Biomarker Detection Technique in Serum/Plasma Samples by Integrating Dielectrophoresis and Fluorescence Based Platform [D] . Logeeshan, Velmanickam 2019

机译：介电电泳与荧光平台整合实现血清/血浆样品中低成本，高通量，高灵敏度的生物标志物检测技术
6. From the Cover: Unraveling the structure of DNA during overstretching by using multicolor single-molecule fluorescence imaging [O] . Joost van Mameren, Peter Gross, Geraldine Farge, 2009

机译：从封面开始：通过使用多色单分子荧光成像来揭示过度拉伸过程中的DNA结构
7. High-throughput platform for real-time monitoring of biological processes by multicolor single-molecule fluorescence [O] . J. Chen, R. V. Dalal, A. N. Petrov, 2013

机译：多色单分子荧光实时监测生物过程的高通量平台

High-throughput platform for real-time monitoring of biological processes by multicolor single-molecule fluorescence

摘要

著录项

相似文献

相关主题

期刊订阅