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首页> 美国卫生研究院文献>Protein Science : A Publication of the Protein Society >The structure of the yrdC gene product from Escherichia coli reveals a new fold and suggests a role in RNA binding.
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The structure of the yrdC gene product from Escherichia coli reveals a new fold and suggests a role in RNA binding.

机译：大肠杆菌的yrdC基因产物的结构揭示了新的折叠并暗示了在RNA结合中的作用。

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摘要

The yrdC family of genes codes for proteins that occur both independently and as a domain in proteins that have been implicated in regulation. An example for the latter case is the sua5 gene from yeast. SuaS was identified as a suppressor of a translation initiation defect in cytochrome c and is required for normal growth in yeast (Na JG, Pinto I, Hampsey M, 1992, Genetics 11:791-801). However, the function of the Sua5 protein remains unknown; Sua5 could act either at the transcriptional or the posttranscriptional levels to compensate for an aberrant translation start codon in the cyc gene. To potentially learn more about the function of YrdC and proteins featuring this domain, the crystal structure of the YrdC protein from Escherichia coli was determined at a resolution of 2.0 A. YrdC adopts a new fold with no obvious similarity to those of other proteins with known three-dimensional (3D) structure. The protein features a large concave surface on one side that exhibits a positive electrostatic potential. The dimensions of this depression, its curvature, and the fact that conserved basic amino acids are located at its floor suggest that YrdC may be a nucleic acid binding protein. An investigation of YrdC's binding affinities for single- and double-stranded RNA and DNA fragments as well as tRNAs demonstrates that YrdC binds preferentially to double-stranded RNA. Our work provides evidence that 3D structures of functionally uncharacterized gene products with unique sequences can yield novel folds and functional insights.
机译：yrdC基因家族编码的蛋白质既独立存在，又作为与调节有关的蛋白质的结构域存在。后一种情况的一个例子是来自酵母的sua5基因。 SuaS被鉴定为细胞色素c的翻译起始缺陷的抑制剂，并且是酵母中正常生长所必需的（Na JG，Pinto I，Hampsey M，1992，Genetics 11：791-801）。然而，Sua5蛋白的功能仍然未知。 Sua5可以在转录水平或转录后水平上起作用，以补偿cyc基因中异常的翻译起始密码子。为了潜在地了解有关YrdC和具有该结构域的蛋白质的功能的更多信息，以2.0 A的分辨率测定了来自大肠杆菌的YrdC蛋白质的晶体结构。三维（3D）结构。该蛋白质的一侧具有大凹面，具有正静电势。该凹陷的大小，曲率以及保守的碱性氨基酸位于其底部的事实表明，YrdC可能是核酸结合蛋白。对YrdC对单链和双链RNA和DNA片段以及tRNA的结合亲和力的研究表明，YrdC优先与双链RNA结合。我们的工作提供了证据，即具有独特序列的功能未知的基因产物的3D结构可产生新颖的折叠和功能洞察力。

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