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首页> 美国卫生研究院文献>Redox Biology >Catalase activity is stimulated by H2O2 in rich culture medium and is required for H2O2 resistance and adaptation in yeast
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Catalase activity is stimulated by H2O2 in rich culture medium and is required for H2O2 resistance and adaptation in yeast

机译：过氧化氢酶的活性被富氧培养基中的过氧化氢刺激是过氧化氢抗性和酵母适应性所必需的

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摘要

Catalases are efficient scavengers of H2O2 and protect cells against H2O2 stress. Examination of the H2O2 stimulon in Saccharomyces cerevisiae revealed that the cytosolic catalase T (Ctt1) protein level increases 15-fold on H2O2 challenge in synthetic complete media although previous work revealed that deletion of the CCT1 or CTA1 genes (encoding peroxisomal/mitochondrial catalase A) does not increase the H2O2 sensitivity of yeast challenged in phosphate buffer (pH 7.4). This we attributed to our observation that catalase activity is depressed when yeast are challenged with H2O2 in nutrient-poor media. Hence, we performed a systematic comparison of catalase activity and cell viability of wild-type yeast and of the single catalase knockouts, ctt1∆ and cta1∆, following H2O2 challenge in nutrient-rich medium (YPD) and in phosphate buffer (pH 7.4). Ctt1 but not Cta1 activity is strongly induced by H2O2 when cells are challenged in YPD but suppressed when cells are challenged in buffer. Consistent with the activity results, exponentially growing ctt1∆ cells in YPD are more sensitive to H2O2 than wild-type or cta1∆ cells, whereas in buffer all three strains exhibit comparable H2O2 hypersensitivity. Furthermore, catalase activity is increased during adaptation to sublethal H2O2 concentrations in YPD but not in buffer. We conclude that induction of cytosolic Ctt1 activity is vital in protecting yeast against exogenous H2O2 but this activity is inhibited by H2O2 when cells are challenged in nutrient-free media.
机译：过氧化氢酶是有效的H2O2清除剂，可保护细胞免受H2O2胁迫。对酿酒酵母中H2O2刺激物的检查表明，在合成完全培养基中，H2O2刺激下胞质过氧化氢酶T（Ctt1）蛋白水平增加了15倍，尽管先前的工作表明删除了CCT1或CTA1基因的缺失（编码过氧化物酶体/线粒体过氧化氢酶A）不会增加在磷酸盐缓冲液（pH 7.4）中攻击的酵母对H2O2的敏感性。这归因于我们的观察，即在营养缺乏的培养基中用H2O2攻击酵母时，过氧化氢酶的活性会降低。因此，我们在营养丰富的培养基（YPD）和磷酸盐缓冲液（pH 7.4）中对H2O2进行攻击后，对野生型酵母和单个过氧化氢酶敲除物ctt1∆和cta1∆的过氧化氢酶活性和细胞活力进行了系统的比较。 。当在YPD中攻击细胞时，H2O2强烈诱导Ctt1而非Cta1活性，而在缓冲液中攻击细胞时，H2O2强烈诱导Ctt1活性。与活性结果一致，YPD中成倍生长的ctt1∆细胞比野生型或cta1∆细胞对H 2 O 2 敏感，而在缓冲液中所有这三种菌株表现出可比的H 2 O 2 超敏性。此外，过氧化氢酶活性在适应YPD中亚致死H 2 O 2 浓度的过程中增加，但在缓冲液中不增加。我们得出的结论是，诱导胞质Ctt1活性对于保护酵母免受外源H 2 O 2 至关重要，但该活性受到H 2 O <当在无营养的培养基中攻击细胞时，则为sub> 2 。

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