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首页> 美国卫生研究院文献>Science and Technology of Advanced Materials >Porous hydroxyapatite and biphasic calcium phosphate ceramics promote ectopic osteoblast differentiation from mesenchymal stem cells
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Porous hydroxyapatite and biphasic calcium phosphate ceramics promote ectopic osteoblast differentiation from mesenchymal stem cells

机译：多孔羟基磷灰石和双相磷酸钙陶瓷促进异位成骨细胞从间充质干细胞分化

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Because calcium phosphate (Ca–P) ceramics have been used as bone substitutes, it is necessary to investigate what effects the ceramics have on osteoblast maturation. We prepared three types of Ca–P ceramics with different Ca–P ratios, i.e. hydroxyapatite (HA), beta-tricalcium phosphate (β-TCP), and biphasic calcium phosphate (BCP) ceramics with dense-smooth and porous structures. Comprehensive gene expression microarray analysis of mouse osteoblast-like cells cultured on these ceramics revealed that porous Ca–P ceramics considerably affected the gene expression profiles, having a higher potential for osteoblast maturation. In the in vivo study that followed, porous Ca–P ceramics were implanted into rat skeletal muscle. Sixteen weeks after the implantation, more alkaline-phosphatase-positive cells were observed in the pores of hydroxyapatite and BCP, and the expression of the osteocalcin gene (an osteoblast-specific marker) in tissue grown in pores was also higher in hydroxyapatite and BCP than in β-TCP. In the pores of any Ca–P ceramics, 16 weeks after the implantation, we detected the expressions of marker genes of the early differentiation stage of chondrocytes and the complete differentiation stage of adipocytes, which originate from mesenchymal stem cells, as well as osteoblasts. These marker gene expressions were not observed in the muscle tissue surrounding the implanted Ca–P ceramics. These observations indicate that porous hydroxyapatite and BCP had a greater potential for promoting the differentiation of mesenchymal stem cells into osteoblasts than β-TCP.
机译：由于磷酸钙（Ca–P）陶瓷已被用作骨替代品，因此有必要研究陶瓷对成骨细胞成熟的影响。我们准备了三种具有不同Ca-P比的Ca-P陶瓷，即羟基磷灰石（HA），β-磷酸三钙（β-TCP）和双相磷酸钙（BCP）陶瓷，它们具有致密光滑和多孔的结构。对在这些陶瓷上培养的小鼠成骨样细胞进行全面的基因表达微阵列分析，发现多孔Ca–P陶瓷极大地影响了基因表达谱，具有更高的成骨细胞成熟潜能。在随后的体内研究中，将多孔Ca-P陶瓷植入大鼠骨骼肌中。植入后16周，在羟基磷灰石和BCP的孔中观察到更多的碱性磷酸酶阳性细胞，并且在羟基磷灰石和BCP的孔中生长的组织中骨钙素基因（成骨细胞特异性标志物）的表达也更高。在β-TCP中。在任何Ca-P陶瓷的孔中，植入后16周，我们检测到了软骨细胞早期分化阶段和脂肪细胞完全分化阶段的标记基因的表达，这些基因起源于间充质干细胞以及成骨细胞。在植入的Ca-P陶瓷周围的肌肉组织中未观察到这些标志物基因的表达。这些观察结果表明，与β-TCP相比，多孔羟基磷灰石和BCP具有更大的潜力促进间充质干细胞向成骨细胞的分化。

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