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首页> 美国卫生研究院文献>The Scientific World Journal >Host Suitability of House Fly Musca domestica (Diptera: Muscidae) Pupae Killed by High or Low Temperature Treatment for a Parastoid Spalangia endius (Hymenoptera: Pteromalidae)
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Host Suitability of House Fly Musca domestica (Diptera: Muscidae) Pupae Killed by High or Low Temperature Treatment for a Parastoid Spalangia endius (Hymenoptera: Pteromalidae)

机译：房蝇家蝇（双翅目：蝇科）P经高温或低温处理杀死的寄生虫Spa虫（膜翅目：蝶翅目）的寄主适合性

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摘要

The objective of this study was to establish a high quality progeny production system for the house fly parasitoid, Spalangia endius (Hymenoptera: Pteromalidae), by stockpiling hosts. We performed two host killing methods before host storage: (i) heat-killed by 30 min exposure to 50°C or (ii) freeze-killed by 10 min exposure to −80°C. The average number of parasitoids that emerged from nonstored house fly pupae after heat- or freeze-killing was not significantly different from live pupae. When house fly pupae stored at −20°C after heat-killing were supplied to S. endius, progeny production was significantly less than live pupae. Moreover, productivity became very low when house fly pupae refrigerated at 3°C after heat- or freeze-killing were supplied to S. endius. On the other hand, when house fly pupae stored at −80°C for 1 year after heat-killing were supplied to S. endius, the average number of parasitoids that emerged was not significantly different from live pupae. The average number of parasitoids that emerged from freeze-killed hosts kept for more than 8 weeks at −80°C was significantly fewer than live pupae. Thus, this study clarified that a higher-quality host can be maintained not only by simply storing at –80°C but also by adding heat treatment before storage.
机译：这项研究的目的是通过贮藏寄主为家蝇的拟寄生物Spalandia endius（膜翅目：翼手龙）建立高质量的后代生产系统。我们在寄主存放之前执行了两种寄主杀死方法：（i）在暴露于50°C的环境中30分钟进行热杀死，或（ii）通过暴露于-80°C的10分钟中进行冷冻杀死。非储存的家蝇p在加热或冷冻杀死后出现的平均寄生虫数量与活p没有显着差异。当将热灭活后储存在-20°C的家蝇p供应给内生链霉菌时，后代的产量显着低于活p。此外，当将杀热或冷冻杀死的after蝇fly在3℃下冷藏后，再给内生链霉菌提供生产力时，其生产率将变得很低。另一方面，当将杀热后在-80℃下保存了一年的家蝇p供应给内生链霉菌时，平均出现的寄生虫数量与活p没有显着差异。从冷冻杀死的宿主在-80°C下保存8周以上的平均寄生虫数量明显少于活p。因此，这项研究澄清说，不仅可以通过简单地在–80°C下保存，而且可以通过在保存之前添加热处理来维持更高质量的宿主。

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