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首页> 美国卫生研究院文献>Sensors (Basel Switzerland) >Electrochemical Determination of Low Molecular Mass Thiols Content in Potatoes (Solanum tuberosum) Cultivated in the Presence of Various Sulphur Forms and Infected by Late Blight (Phytophora infestans)
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Electrochemical Determination of Low Molecular Mass Thiols Content in Potatoes (Solanum tuberosum) Cultivated in the Presence of Various Sulphur Forms and Infected by Late Blight (Phytophora infestans)

机译：电化学测定存在多种硫形式并受晚疫病（致病疫霉）感染的马铃薯（马铃薯）中低分子量硫醇的含量

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In the present paper potato plants were cultivated in the presence of ammonium sulphate or elemental sulphur supplementation into the soil to reveal the effects of different sulphur forms on content of nitrogen, phosphorus, potassium, calcium, magnesium and sulphur, and yield of tubers. During the investigation of the influence of different sulphur forms on yield of potato tubers we did not observe significant changes. Average weight of tubers of control plants per one experimental pot was 355 g. Application of sulphur in both forms resulted in moderate potato tubers weight reduction per one experimental pot compared to control group; average value ranged from 320 to 350 g per one experimental pot. Further we treated the plants with two different supplementation of sulphur with cadmium(II) ions (4 mg of cadmium(II) acetate per kilogram of the soil). The significantly lowest cadmium content (p < 0.05) was determined in tissues of plants treated with the highest dosage of elemental sulphur (0.64 mg Cd/kg) compared to control plants (0.82 mg Cd/kg). We also aimed our attention on the cadmium content in proteins, lipids or soluble carbohydrates and ash. Application of sulphate as well as elemental sulphur resulted in significant cadmium content reduction in lipid fraction compared to control plants. In addition to this we quantified content of low molecular mass thiols in potatoes tissues. To determine the thiols content we employed differential pulse voltammetry Brdicka reaction. After twelve days of the treatment enhancing of thiols level was observed in all experimental groups regardless to applied sulphur form and its concentration. Finally we evaluated the effect of sulphur supplementation on Phytophora infestans infection of potato plants.
机译：在本文中，马铃薯植物在硫酸铵或元素硫的存在下种植到土壤中，以揭示不同形式的硫对氮，磷，钾，钙，镁和硫的含量以及块茎产量的影响。在调查不同硫形式​​对马铃薯块茎产量的影响期间，我们没有观察到明显的变化。每一个实验盆中对照植物的块茎平均重量为355 g。与对照组相比，以两种形式施用硫磺可使每个实验盆的马铃薯块茎轻度减轻。每个实验罐的平均值介于320至350克之间。此外，我们用两种不同的硫补充镉（II）离子（每千克土壤4毫克乙酸镉（II））对植物进行了处理。与对照植物（0.82 mg Cd / kg）相比，用最高剂量的元素硫（0.64 mg Cd / kg）处理过的植物组织中的镉含量最低（p <0.05）。我们还将注意力集中在蛋白质，脂质或可溶性碳水化合物和灰分中的镉含量上。与对照植物相比，硫酸盐和元素硫的施用显着降低了脂质部分的镉含量。除此之外，我们还定量了马铃薯组织中低分子量硫醇的含量。为了确定硫醇含量，我们采用差分脉冲伏安法Brdicka反应。处理十二天后，无论施用的硫形式及其浓度如何，在所有实验组中均观察到硫醇水平的提高。最后，我们评估了硫磺添加对马铃薯植株晚疫病感染的影响。

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