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首页> 美国卫生研究院文献>Sensors (Basel Switzerland) >Deployment of a Prototype Plant GFP Imager at the Arthur Clarke Mars Greenhouse of the Haughton Mars Project
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Deployment of a Prototype Plant GFP Imager at the Arthur Clarke Mars Greenhouse of the Haughton Mars Project

机译：在Haughton Mars项目的Arthur Clarke Mars温室部署原型植物GFP成像仪

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The use of engineered plants as biosensors has made elegant strides in the past decades, providing keen insights into the health of plants in general and particularly in the nature and cellular location of stress responses. However, most of the analytical procedures involve laboratory examination of the biosensor plants. With the advent of the green fluorescence protein (GFP) as a biosensor molecule, it became at least theoretically possible for analyses of gene expression to occur telemetrically, with the gene expression information of the plant delivered to the investigator over large distances simply as properly processed fluorescence images. Spaceflight and other extraterrestrial environments provide unique challenges to plant life, challenges that often require changes at the gene expression level to accommodate adaptation and survival. Having previously deployed transgenic plant biosensors to evaluate responses to orbital spaceflight, we wished to develop the plants and especially the imaging devices required to conduct such experiments robotically, without operator intervention, within extraterrestrial environments. This requires the development of an autonomous and remotely operated plant GFP imaging system and concomitant development of the communications infrastructure to manage dataflow from the imaging device. Here we report the results of deploying a prototype GFP imaging system within the Arthur Clarke Mars Greenhouse (ACMG) an autonomously operated greenhouse located within the Haughton Mars Project in the Canadian High Arctic. Results both demonstrate the applicability of the fundamental GFP biosensor technology and highlight the difficulties in collecting and managing telemetric data from challenging deployment environments.
机译：在过去的几十年中，将工程植物用作生物传感器已取得了长足的进步，使人们对植物的整体健康，尤其是胁迫反应的性质和细胞位置有了深入的了解。但是，大多数分析程序都涉及对生物传感器工厂的实验室检查。随着绿色荧光蛋白（GFP）作为生物传感器分子的出现，至少在理论上可以遥测地进行基因表达的分析，而将植物的基因表达信息以适当的方式简单地长距离传递给研究者荧光图像。航天和其他地球外环境给植物生命带来了独特的挑战，这些挑战通常需要在基因表达水平上进行改变以适应适应和生存。先前已经部署了转基因植物生物传感器来评估对轨道太空飞行的反应，我们希望开发植物，尤其是在地外环境中无需操作员干预即可自动进行此类实验的成像设备。这需要开发自主和远程操作的植物GFP成像系统，并伴随开发通信基础设施以管理来自成像设备的数据流。在这里，我们报告了在Arthur Clarke Mars温室（ACMG）中部署原型GFP成像系统的结果，该温室是位于加拿大高北极地区Haughton Mars项目内的一个自主经营的温室。结果既证明了基本的GFP生物传感器技术的适用性，又突显了在具有挑战性的部署环境中收集和管理遥测数据的困难。

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