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首页> 美国卫生研究院文献>Sensors (Basel Switzerland) >Passive Fluidic Chip Composed of Integrated Vertical Capillary Tubes Developed for On-Site SPR Immunoassay Analysis Targeting Real Samples
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Passive Fluidic Chip Composed of Integrated Vertical Capillary Tubes Developed for On-Site SPR Immunoassay Analysis Targeting Real Samples

机译：专为现场样品开发的现场SPR免疫分析开发的集成垂直毛细管的被动流体芯片

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摘要

We have successfully developed a surface plasmon resonance (SPR) measurement system for the on-site immunoassay of real samples. The system is composed of a portable SPR instrument (290 mm(W) × 160 mm(D) × 120 mm(H)) and a microfluidic immunoassay chip (16 mm(W) × 16 mm(D) × 4 mm(H)) that needs no external pump system. An integrated vertical capillary tube functions as a large volume (150 μL) passive pump and a waste reservoir that has sufficient capacity for several refill operations. An immunoassay was carried out that employed the direct injection of a buffer and a test sample in sequence into a microfluidic chip that included 9 antibody bands and 10 reference reagent bands immobilized in the flow channel. By subtracting a reliable averaged reference sensorgram from the antibody, we effectively reduced the influence of the non-specific binding, and then our chip successfully detected the specific binding of spiked IgG in non-homogeneous milk. IgG is a model antigen that is certain not to be present in non-homogeneous milk, and non-homogeneous milk is a model of real sample that includes many interfering foreign substances that induce non-specific binding. The direct injection of a real sample with no pretreatment enabled us to complete the entire immunoassay in several minutes. This ease of operation and short measuring time are acceptable for on-site agricultural, environmental and medical testing.
机译：我们已经成功开发了一种表面等离振子共振（SPR）测量系统，用于对真实样品进行现场免疫测定。该系统由便携式SPR仪器（290 mm（W）×160 mm（D）×120 mm（H））和微流体免疫测定芯片（16 mm（W）×16 mm（D）×4 mm（H））组成）），不需要外部泵系统。集成的垂直毛细管可充当大容量（150μL）的被动泵和废物储存器，该废物储存器具有足够的容量用于多次填充操作。进行了一种免疫测定，该测定采用将缓冲液和测试样品按顺序直接注入微流控芯片中的方法，该芯片包括固定在流道中的9条抗体带和10条参考试剂带。通过从抗体中减去可靠的平均参考传感图，我们有效地减少了非特异性结合的影响，然后我们的芯片成功检测了非均质牛奶中加标IgG的特异性结合。 IgG是一种模型抗原，肯定不存在于非均质牛奶中，而非均质牛奶则是真实样品的模型，其中包括许多诱导非特异性结合的干扰性异物。直接进样而无需预处理的真实样品使我们能够在几分钟内完成整个免疫测定。这种简便的操作和较短的测量时间可用于现场农业，环境和医学测试。

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