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Wireless and Simultaneous Detections of Multiple Bio-Molecules in a Single Sensor Using Love Wave Biosensor

机译:使用Love Wave生物传感器在单个传感器中无线同时检测多个生物分子

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摘要

A Love wave-based biosensor with a 440 MHz center frequency was developed for the simultaneous detection of two different analytes of Cartilage Oligomeric Matrix Protein (COMP) and rabbit immunoglobulin G (IgG) in a single sensor. The developed biosensor consists of one-port surface acoustic wave (SAW) reflective delay lines on a 41° YX LiNbO3 piezoelectric substrate, a poly(methyl methacrylate) (PMMA) waveguide layer, and two different sensitive films. The Love wave biosensor was wirelessly characterized using two antennas and a network analyzer. The binding of the analytes to the sensitive layers induced a large change in the time positions of the original reflection peaks mainly due to the mass loading effect. The assessed time shifts in the reflection peaks were matched well with the predicted values from coupling of mode (COM) modeling. The sensitivities evaluated from the sensitive films were ∼15 deg/μg/mL for the rabbit IgG and ∼1.8 degg/mL for COMP.
机译:开发了基于爱波的中心频率为440 MHz的生物传感器,用于在单个传感器中同时检测软骨低聚基质蛋白(COMP)和兔免疫球蛋白G(IgG)的两种不同分析物。研发的生物传感器由41°YX LiNbO3压电基板上的单端口表面声波(SAW)反射延迟线,聚甲基丙烯酸甲酯(PMMA)波导层和两个不同的敏感膜组成。 Love波生物传感器使用两个天线和一个网络分析仪进行无线表征。主要由于质量加载效应,分析物与敏感层的结合导致原始反射峰的时间位置发生较大变化。反射峰的评估时移与模式耦合(COM)建模的预测值非常匹配。从敏感膜上测得的灵敏度对兔子IgG约为15度/微克/毫升,对COMP而言约为1.8度/纳克/毫升。

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