• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>Sensors (Basel Switzerland) >Development and Characterization of a Diamond-Insulated Graphitic Multi Electrode Array Realized with Ion Beam Lithography
【2h】

Development and Characterization of a Diamond-Insulated Graphitic Multi Electrode Array Realized with Ion Beam Lithography

机译：离子束光刻技术实现金刚石绝缘石墨多电极阵列的研制与表征

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The detection of quantal exocytic events from neurons and neuroendocrine cells is a challenging task in neuroscience. One of the most promising platforms for the development of a new generation of biosensors is diamond, due to its biocompatibility, transparency and chemical inertness. Moreover, the electrical properties of diamond can be turned from a perfect insulator into a conductive material (resistivity ∼mΩ·cm) by exploiting the metastable nature of this allotropic form of carbon. A 16-channels MEA (Multi Electrode Array) suitable for cell culture growing has been fabricated by means of ion implantation. A focused 1.2 MeV He+ beam was scanned on a IIa single-crystal diamond sample (4.5 × 4.5 × 0.5 mm3) to cause highly damaged sub-superficial structures that were defined with micrometric spatial resolution. After implantation, the sample was annealed. This process provides the conversion of the sub-superficial highly damaged regions to a graphitic phase embedded in a highly insulating diamond matrix. Thanks to a three-dimensional masking technique, the endpoints of the sub-superficial channels emerge in contact with the sample surface, therefore being available as sensing electrodes. Cyclic voltammetry and amperometry measurements of solutions with increasing concentrations of adrenaline were performed to characterize the biosensor sensitivity. The reported results demonstrate that this new type of biosensor is suitable for in vitro detection of catecholamine release.
机译：从神经元和神经内分泌细胞中检测定量的胞外事件是神经科学中一项艰巨的任务。金刚石由于具有生物相容性，透明度和化学惰性，因此是开发新一代生物传感器的最有希望的平台之一。此外，通过利用碳的同素异形形式的亚稳态性质，可以将金刚石的电性能从理想的绝缘体转变为导电材料（电阻率〜mΩ·cm）。通过离子注入已经制造出适合于细胞培养生长的16通道MEA（多电极阵列）。在IIa单晶金刚石样品（4.5×4.5×0.5 mm 3 ）上扫描聚焦的1.2 MeV He + 光束，造成高度损坏的亚表层结构。用微米空间分辨率定义。植入后，将样品退火。该过程提供了将亚表层高度损坏的区域转换为嵌入在高度绝缘的金刚石基质中的石墨相的功能。由于采用了三维掩蔽技术，子浅通道的端点与样品表面接触，因此可以用作传感电极。对肾上腺素浓度增加的溶液进行循环伏安法和安培法测量，以表征生物传感器的敏感性。报道的结果表明，这种新型的生物传感器适用于体外检测儿茶酚胺的释放。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号