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首页> 美国卫生研究院文献>Sensors (Basel Switzerland) >Utilisation of Quartz Crystal Microbalance Sensors with Dissipation (QCM-D) for a Clauss Fibrinogen Assay in Comparison with Common Coagulation Reference Methods
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Utilisation of Quartz Crystal Microbalance Sensors with Dissipation (QCM-D) for a Clauss Fibrinogen Assay in Comparison with Common Coagulation Reference Methods

机译：耗散的石英晶体微量天平传感器（QCM-D）用于克劳斯纤维蛋白原测定的效果与常见凝血参考方法的比较

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The determination of fibrinogen levels is one of the most important coagulation measurements in medicine. It plays a crucial part in diagnostic and therapeutic decisions, often associated with time-critical conditions. The commonly used measurement is the Clauss fibrinogen assay (CFA) where plasma is activated by thrombin reagent and which is conducted by mechanical/turbidimetric devices. As quartz crystal microbalance sensors with dissipation (QCM-D) based devices have a small footprint, can be operated easily and allow measurements independently from sample transportation time, laboratory location, availability and opening hours, they offer a great opportunity to complement laboratory CFA measurements. Therefore, the objective of the work was to (1) transfer the CFA to the QCM-D method; (2) develop an easy, time- and cost-effective procedure and (3) compare the results with references. Different sensor coatings (donor’s own plasma; gold surface) and different QCM-D parameters (frequency signal shift; its calculated turning point; dissipation signal shift) were sampled. The results demonstrate the suitability for a QCM-D-based CFA in physiological fibrinogen ranges. Results were obtained in less than 1 min and in very good agreement with a standardized reference (Merlin coagulometer). The results provide a good basis for further investigation and pave the way to a possible application of QCM-D in clinical and non-clinical routine in the medical field.
机译：纤维蛋白原水平的测定是医学上最重要的凝血指标之一。它在诊断和治疗决策中起着至关重要的作用，通常与时效性疾病相关。常用的测量方法是克劳斯纤维蛋白原测定法（CFA），其中血浆通过凝血酶试剂激活，并通过机械/比浊仪进行。由于基于耗散（QCM-D）的石英晶体微天平传感器的占地面积小，易于操作并且可以独立于样品运输时间，实验室位置，可用性和开放时间进行测量，因此它们为补充实验室CFA测量提供了绝佳机会。因此，工作的目的是（1）将CFA转换为QCM-D方法； （2）开发一种简单，省时且具有成本效益的程序，（3）将结果与参考进行比较。对不同的传感器涂层（供体自身的等离子体；金表面）和不同的QCM-D参数（频率信号偏移；其计算出的转折点；耗散信号偏移）进行了采样。结果表明在生理性纤维蛋白原范围内适用于基于QCM-D的CFA。在不到1分钟的时间内获得了结果，并且与标准参考值（Merlin凝血计）非常吻合。该结果为进一步研究提供了良好的基础，并为QCM-D在医学领域的临床和非临床常规应用中的可能应用铺平了道路。

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