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首页> 美国卫生研究院文献>Sensors (Basel Switzerland) >Development of a Sensitive Electrochemical Enzymatic Reaction-Based Cholesterol Biosensor Using Nano-Sized Carbon Interdigitated Electrodes Decorated with Gold Nanoparticles
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Development of a Sensitive Electrochemical Enzymatic Reaction-Based Cholesterol Biosensor Using Nano-Sized Carbon Interdigitated Electrodes Decorated with Gold Nanoparticles

机译：金纳米粒子修饰的纳米碳指叉电极开发基于电化学反应的胆固醇生物传感器

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We developed a versatile and highly sensitive biosensor platform. The platform is based on electrochemical-enzymatic redox cycling induced by selective enzyme immobilization on nano-sized carbon interdigitated electrodes (IDEs) decorated with gold nanoparticles (AuNPs). Without resorting to sophisticated nanofabrication technologies, we used batch wafer-level carbon microelectromechanical systems (C-MEMS) processes to fabricate 3D carbon IDEs reproducibly, simply, and cost effectively. In addition, AuNPs were selectively electrodeposited on specific carbon nanoelectrodes; the high surface-to-volume ratio and fast electron transfer ability of AuNPs enhanced the electrochemical signal across these carbon IDEs. Gold nanoparticle characteristics such as size and morphology were reproducibly controlled by modulating the step-potential and time period in the electrodeposition processes. To detect cholesterol selectively using AuNP/carbon IDEs, cholesterol oxidase (ChOx) was selectively immobilized via the electrochemical reduction of the diazonium cation. The sensitivity of the AuNP/carbon IDE-based biosensor was ensured by efficient amplification of the redox mediators, ferricyanide and ferrocyanide, between selectively immobilized enzyme sites and both of the combs of AuNP/carbon IDEs. The presented AuNP/carbon IDE-based cholesterol biosensor exhibited a wide sensing range (0.005–10 mM) and high sensitivity (~993.91 µA mM−1 cm−2; limit of detection (LOD) ~1.28 µM). In addition, the proposed cholesterol biosensor was found to be highly selective for the cholesterol detection.
机译：我们开发了一个多功能且高度敏感的生物传感器平台。该平台基于通过选择性酶固定在装饰有金纳米颗粒（AuNPs）的纳米级碳指叉电极（IDE）上诱导的电化学-酶氧化还原循环。在不依靠复杂的纳米制造技术的情况下，我们使用批处理晶圆级碳微机电系统（C-MEMS）工艺可重复，简单且经济高效地制造3D碳IDE。此外，AuNPs被选择性地电沉积在特定的碳纳米电极上。 AuNPs的高体积比和快速的电子转移能力增强了跨这些碳IDE的电化学信号。通过调节电沉积过程中的阶跃电位和时间段，可重复控制金纳米粒子的特性，例如大小和形态。为了使用AuNP /碳IDE选择性地检测胆固醇，通过重氮阳离子的电化学还原选择性地固定了胆固醇氧化酶（ChOx）。基于AuNP / carbon IDE的生物传感器的敏感性通过选择性固定的酶位点与AuNP / carbon IDE的两个梳齿之间的氧化还原介体，铁氰化物和亚铁氰化物的有效扩增来确保。提出的基于AuNP /碳IDE的胆固醇生物传感器具有宽的感测范围（0.005–10 mM）和高的灵敏度（〜993.91 µA mM -1 cm -2 ；极限检测量（LOD）〜1.28 µM）。另外，发现提出的胆固醇生物传感器对胆固醇检测具有高度选择性。

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