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首页> 美国卫生研究院文献>Sensors (Basel Switzerland) >Development and Validation of an On-Line Water Toxicity Sensor with Immobilized Luminescent Bacteria for On-Line Surface Water Monitoring
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Development and Validation of an On-Line Water Toxicity Sensor with Immobilized Luminescent Bacteria for On-Line Surface Water Monitoring

机译：用于地面水在线监测的带有固定化发光细菌的在线水毒性传感器的开发和验证

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Surface water used for drinking water production is frequently monitored in The Netherlands using whole organism biomonitors, with for example Daphnia magna or Dreissena mussels, which respond to changes in the water quality. However, not all human-relevant toxic compounds can be detected by these biomonitors. Therefore, a new on-line biosensor has been developed, containing immobilized genetically modified bacteria, which respond to genotoxicity in the water by emitting luminescence. The performance of this sensor was tested under laboratory conditions, as well as under field conditions at a monitoring station along the river Meuse in The Netherlands. The sensor was robust and easy to clean, with inert materials, temperature control and nutrient feed for the reporter organisms. The bacteria were immobilized in sol-gel on either an optical fiber or a glass slide and then continuously exposed to water. Since the glass slide was more sensitive and robust, only this setup was used in the field. The sensor responded to spikes of genotoxic compounds in the water with a minimal detectable concentration of 0.01 mg/L mitomycin C in the laboratory and 0.1 mg/L mitomycin C in the field. With further optimization, which should include a reduction in daily maintenance, the sensor has the potential to become a useful addition to the currently available biomonitors.
机译：在荷兰，经常使用全生物生物监测仪监测饮用水生产所用的地表水，例如，对水质变化做出反应的大型蚤（Daphnia magna或Dreissena贻贝）。但是，这些生物监控器无法检测到所有与人类有关的有毒化合物。因此，已经开发了一种新的在线生物传感器，其包含固定的转基因细菌，该细菌通过发射发光来响应水中的遗传毒性。该传感器的性能在实验室条件下以及在荷兰默兹河沿岸的监测站的现场条件下进行了测试。该传感器坚固耐用，易于清洁，具有用于报告生物的惰性材料，温度控制和营养饲料。将细菌固定在光纤或载玻片上的溶胶-凝胶中，然后连续暴露于水。由于载玻片更加灵敏和坚固，因此在现场仅使用此设置。传感器对水中的遗传毒性化合物的尖峰有反应，实验室中最低检出浓度为0.01 mg / L丝裂霉素C，现场最低检出浓度为0.1 mg / L丝裂霉素C。通过进一步的优化（包括减少日常维护），该传感器有可能成为当前可用生物监控器的有用补充。

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