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首页> 美国卫生研究院文献>Sensors (Basel Switzerland) >Amphiphilic Fluorine-Containing Block Copolymers as Carriers for Hydrophobic PtTFPP for Dissolved Oxygen Sensing Cell Respiration Monitoring and In Vivo Hypoxia Imaging with High Quantum Efficiency and Long Lifetime
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Amphiphilic Fluorine-Containing Block Copolymers as Carriers for Hydrophobic PtTFPP for Dissolved Oxygen Sensing Cell Respiration Monitoring and In Vivo Hypoxia Imaging with High Quantum Efficiency and Long Lifetime

机译：两亲性含氟嵌段共聚物作为疏水性PtTFPP的载体用于溶解氧传感细胞呼吸监测和体内缺氧成像具有高量子效率和长寿命

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New amphiphilic star or multi-arm block copolymers with different structures were synthesized for enabling the use of hydrophobic oxygen probe of platinum (II)-tetrakis (pentafluorophenyl) porphyrin (PtTFPP) for bioanalysis. The amphiphilic star polymers were prepared through the Atom Transfer Radical Polymerization (ATRP) method by using hydrophilic 4-arm polyethylene glycol (4-arm-PEG) as an initiator. Among the five block copolymers, >P1 series (>P1a, >P1b, and >P1c) and >P3 possess fluorine-containing moieties to improve the oxygen sensitivity with its excellent capacity to dissolve and carry oxygen. A polymer >P2 without fluorine units was also synthesized for comparison. The structure-property relationship was investigated. Under nitrogen atmosphere, high quantum efficiency of PtTFPP in fluorine-containing micelles could reach to 22% and long lifetime could reach to 76 μs. One kind of representative PtTFPP-containing micelles was used to detect the respiration of Escherichia coli (E. coli) JM109 and macrophage cell J774A.1 by a high throughput plate reader. In vivo hypoxic imaging of tumor-bearing mice was also achieved successfully. This study demonstrated that using well-designed fluoropolymers to load PtTFPP could achieve high oxygen sensing properties, and long lifetime, showing the great capability for further in vivo sensing and imaging.
机译：合成了具有不同结构的新型两亲星形或多臂嵌段共聚物，以便能够使用铂（II）-四（五氟苯基）卟啉（PtTFPP）的疏水性氧探针进行生物分析。通过使用亲水性4-臂聚乙二醇（4-臂-PEG）作为引发剂，通过原子转移自由基聚合（ATRP）方法制备两亲星形聚合物。在这五种嵌段共聚物中，> P1 系列（> P1a ，> P1b 和> P1c ）和> P3 < / strong>具有含氟部分，以其出色的溶解和携带氧气的能力来提高氧气的敏感性。还合成了不含氟单元的聚合物> P2 用于比较。研究了结构-属性关系。在氮气氛下，PtTFPP在含氟胶束中的高量子效率可达22％，长寿命可达76μs。一种具有代表性的含PtTFPP的胶束通过高通量读板器用于检测大肠杆菌（E. coli）JM109和巨噬细胞J774A.1的呼吸。还成功实现了荷瘤小鼠的体内低氧成像。这项研究表明，使用设计良好的含氟聚合物加载PtTFPP可以实现较高的氧传感性能，并具有较长的使用寿命，显示出进一步体内传感和成像的强大能力。

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