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Non-Contact Temperature Control System Applicable to Polymerase Chain Reaction on a Lab-on-a-Disc

机译:非接触式温度控制系统适用于光盘实验室中的聚合酶链反应

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摘要

Polymerase chain reaction (PCR) and the visual inspection of fluorescent amplicons for detection are commonly used procedures in nucleic acid tests. However, it has been extremely challenging to incorporate PCR onto a lab-on-a-disc (PCR–LOD) as it involves controlling the complicated and precise heating steps during thermal cycling and the measurement of reagent temperature. Additionally, a non-contact temperature control system without any connecting attachments needs to be implemented to facilitate the rotation of the PCR–LOD. This study presents a non-contact temperature control system to integrate conventional PCR onto an LOD. The experimental results demonstrate that our proposed system provides one-stop detection capabilities for Salmonella with a stable PCR amplification in a single PCR–LOD.
机译:聚合酶链反应(PCR)和荧光扩增子的目视检查以进行检测是核酸测试中常用的程序。但是,将PCR整合到盘上实验室(PCR-LOD)上具有极大的挑战性,因为它涉及到控制热循环过程中复杂而精确的加热步骤以及试剂温度的测量。此外,需要实施无任何连接附件的非接触式温度控制系统,以方便PCR-LOD的旋转。这项研究提出了一种非接触式温度控制系统,可将常规PCR集成到LOD上。实验结果表明,我们提出的系统在单一PCR-LOD中具有稳定的PCR扩增能力,可为沙门氏菌提供一站式检测功能。

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