• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>Journal of Visualized Experiments : JoVE >A Novel Three-dimensional Flow Chamber Device to Study Chemokine-directed Extravasation of Cells Circulating under Physiological Flow Conditions
【2h】

A Novel Three-dimensional Flow Chamber Device to Study Chemokine-directed Extravasation of Cells Circulating under Physiological Flow Conditions

机译：一种新型的三维流动室装置用于研究在生理流动条件下循环细胞趋化因子导向的外渗

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Extravasation of circulating cells from the bloodstream plays a central role in many physiological and pathophysiological processes, including stem cell homing and tumor metastasis. The three-dimensional flow chamber device (hereafter the 3D device) is a novel in vitro technology that recreates physiological shear stress and allows each step of the cell extravasation cascade to be quantified. The 3D device consists of an upper compartment in which the cells of interest circulate under shear stress, and a lower compartment of static wells that contain the chemoattractants of interest. The two compartments are separated by porous inserts coated with a monolayer of endothelial cells (EC). An optional second insert with microenvironmental cells of interest can be placed immediately beneath the EC layer. A gas exchange unit allows the optimal CO2 tension to be maintained and provides an access point to add or withdraw cells or compounds during the experiment. The test cells circulate in the upper compartment at the desired shear stress (flow rate) controlled by a peristaltic pump. At the end of the experiment, the circulating and migrated cells are collected for further analyses. The 3D device can be used to examine cell rolling on and adhesion to EC under shear stress, transmigration in response to chemokine gradients, resistance to shear stress, cluster formation, and cell survival. In addition, the optional second insert allows the effects of crosstalk between EC and microenvironmental cells to be examined. The translational applications of the 3D device include testing of drug candidates that target cell migration and predicting the in vivo behavior of cells after intravenous injection. Thus, the novel 3D device is a versatile and inexpensive tool to study the molecular mechanisms that mediate cellular extravasation.
机译：从血液中渗出循环细胞在许多生理和病理生理过程（包括干细胞归巢和肿瘤转移）中起着核心作用。三维流动室设备（以下称3D设备）是一种新颖的体外技术，可再现生理切应力并允许量化细胞外渗级联反应的每个步骤。 3D设备包括一个上层隔室，其中感兴趣的细胞在剪切应力下在其中循环，以及一个静态井的下层隔室，其中包含感兴趣的化学吸引剂。这两个隔室被涂覆有单层内皮细胞​​（EC）的多孔插入物隔开。可将带有感兴趣的微环境细胞的第二个可选插入物直接放置在EC层下方。气体交换单元可保持最佳的CO2张力，并提供在实验过程中添加或提取细胞或化合物的入口。测试细胞以蠕动泵控制的所需剪切应力（流速）在上部隔室中循环。在实验结束时，收集循环中和迁移的细胞用于进一步分析。该3D设备可用于检查细胞在剪切应力下的滚动和对EC的粘附，响应趋化因子梯度的迁移，对剪切应力的抵抗力，簇形成和细胞存活。另外，可选的第二插入物允许检查EC和微环境细胞之间的串扰效应。 3D设备的翻译应用程序包括测试靶向细胞迁移的候选药物，并预测静脉注射后细胞的体内行为。因此，新颖的3D设备是研究介导细胞外渗的分子机制的一种通用且廉价的工具。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号