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Complete genome sequence analysis of Archaeoglobus fulgidus strain 7324 (DSM 8774) a hyperthermophilic archaeal sulfate reducer from a North Sea oil field

机译:来自北海油田的超嗜热古生硫酸盐还原剂古细菌古菌7324(DSM 8774)的完整基因组序列分析

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摘要

Archaeoglobus fulgidus is the type species of genus Archaeoglobus Stetter 1998, a hyperthermophilic sulfate reducing group within the Archaeoglobi class of the euryarchaeota phylum. Members of this genus grow heterotrophically or chemolithoautotrophically with sulfate or thiosulfate as electron acceptors. Except for A. fulgidus strain 7324 and the candidate species “Archaeoglobus lithotrophicus”, which both originate from deep oil-fields, the other members of this genus have been recovered from marine hydrothermal systems. Here we describe the features of the A. fulgidus strain 7324 genome as compared to the A. fulgidus VC16 type strain. The 2.3 Mbp genome sequence of strain 7324 shares about 93.5% sequence identity with that of strain VC16T but is about 138 Kbp longer, which is mostly due to two large ‘insertions’ carrying one extra cdc6 (cell-cycle control protein 6) gene, extra CRISPR elements and mobile genetic elements, a high-GC ncRNA gene (hgcC) and a large number of hypothetical gene functions. A comparison with four other Archaeoglobus spp. genomes identified 1001 core Archaeoglobus genes and more than 2900 pan-genome orthologous genes.
机译:富古细菌是古细菌Stetter 1998的类型物种,属于嗜热古菌类古细菌类中的一种超高温硫酸盐还原基团。该属的成员以硫酸盐或硫代硫酸盐作为电子受体异养或化学自养生长。除了都来自深部油田的fulgidus菌株7324和候选物种“ Archaeoglobus lithotrophicus”之外,该属的其他成员已从海洋热液系统中回收。在这里,我们描述了与A.fulgidus VC16型菌株相比,A.fulgidus菌株7324基因组的特征。 7324菌株的2.3 Mbp基因组序列与VC16 T 菌株具有约93.5%的序列同一性,但比VC16 T 的序列长约138 Kbp,这主要是由于两个大的“插入”携带一个额外的cdc6(周期控制蛋白6)基因,额外的CRISPR元件和移动遗传元件,高GC ncRNA基因(hgcC)和大量假设的基因功能。与其他四个古球菌的比较。基因组确定了1001个核心古生球基因和2900个全基因组直系同源基因。

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