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首页> 美国卫生研究院文献>Stem Cells International >Effects of Macromolecular Crowding on Human Adipose Stem Cell Culture in Fetal Bovine Serum Human Serum and Defined Xeno-Free/Serum-Free Conditions
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Effects of Macromolecular Crowding on Human Adipose Stem Cell Culture in Fetal Bovine Serum Human Serum and Defined Xeno-Free/Serum-Free Conditions

机译：大分子拥挤对胎牛血清人血清和确定的无异种/无血清条件下人脂肪干细胞培养的影响

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摘要

Microenvironment plays an important role for stem cell proliferation and differentiation. Macromolecular crowding (MMC) was recently shown to assist stem cells in forming their own matrix microenvironment in vitro. The ability of MMC to support adipose stem cell (ASC) proliferation, metabolism, and multilineage differentiation was studied under different conditions: fetal bovine serum- (FBS-) and human serum- (HS-) based media and xeno- and serum-free (XF/SF) media. Furthermore, the immunophenotype of ASCs under MMC was evaluated. The proliferative capacity of ASCs under MMC was attenuated in each condition. However, osteogenic differentiation was enhanced under MMC, shown by increased deposition of mineralized matrix in FBS and HS cultures. Likewise, significantly greater lipid droplet accumulation and increased collagen IV deposition indicated enhanced adipogenesis under MMC in FBS and HS cultures. In contrast, chondrogenic differentiation was attenuated in ASCs expanded under MMC. The ASC immunophenotype was maintained under MMC with significantly higher expression of CD54. However, MMC impaired metabolic activity and differentiation capacity of ASCs in XF/SF conditions. Both the supportive and inhibitory effects of MMC on ASC are culture condition dependent. In the presence of serum, MMC maintains ASC immunophenotype and enhances adipogenic and osteogenic differentiation at the cost of reduced proliferation.
机译：微环境在干细胞增殖和分化中起着重要作用。最近显示大分子拥挤（MMC）可协助干细胞在体外形成其自身的基质微环境。在以下条件下研究了MMC支持脂肪干细胞（ASC）增殖，代谢和多系分化的能力：胎牛血清（FBS-）和人血清（HS-）培养基以及异种和无血清（XF / SF）媒体。此外，评估了MMC下ASC的免疫表型。在每种情况下，MMC下ASC的增殖能力都会减弱。然而，MMC下成骨分化增强了，在FBS和HS培养物中矿化基质的沉积增加。同样，在FBS和HS培养物中，MMC下脂滴累积明显增加，胶原IV沉积增加，表明脂肪形成增加。相比之下，在MMC下扩展的ASC中，软骨生成的分化减弱。在MMC下维持ASC免疫表型，CD54表达明显升高。但是，MMC在XF / SF条件下会损害ASC的代谢活性和分化能力。 MMC对ASC的支持和抑制作用均取决于培养条件。在存在血清的情况下，MMC维持ASC免疫表型，并以降低增殖为代价增强脂肪形成和成骨分化。

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