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首页> 美国卫生研究院文献>Theranostics >Identification of Volatile Biomarkers of Gastric Cancer Cells and Ultrasensitive Electrochemical Detection based on Sensing Interface of Au-Ag Alloy coated MWCNTs
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Identification of Volatile Biomarkers of Gastric Cancer Cells and Ultrasensitive Electrochemical Detection based on Sensing Interface of Au-Ag Alloy coated MWCNTs

机译：基于Au-Ag合金包覆的MWCNTs传感界面的胃癌细胞挥发性生物标志物的识别和超灵敏电化学检测

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Successful development of novel electrochemical biosensing interface for ultrasensitive detection of volatile biomarkers of gastric cancer cells is a challenging task. Herein we reported to screen out novel volatile biomarkers associated with gastric cancer cells and develop a novel Au-Ag alloy composites-coated MWCNTs as sensing interface for ultrasensitive detection of volatile biomarkers. MGC-803 gastric cancer cells and GES-1 gastric mucous cells were cultured in serum-free media. The sample preparation approaches and HS-SPME conditions were optimized for screening volatile biomarkers. Volatiles emitted from the headspace of the cells/medium culture were identified using GC-MS. The Au-Ag nanoparticles-coated multiwalled carbon nanotubes were prepared as a sensing interface for detection of volatile biomarkers. Results showed that eight different volatile metabolites were screened out between MGC-803 cells and GES-1 cells. Two compounds such as 3-octanone and butanone were specifically present in the headspace of the MGC-803 cells. Three volatiles such as 4-isopropoxybutanol, nonanol and 4-butoxy 1-butanol coexisted in the headspace of both the MGC-803 cells and the GES-1 cells, their concentrations in the headspace of the GES-1cells were markedly higher than those in the MGC-803 cells, three volatiles such as formic acid propyl ester, 1.4-butanediol and 2, 6, 11-trimethyl dodecane solely existed in the headspace of the GES-1 cells. The nanocomposites of MWNTs loaded with Au-Ag nanoparticles were prepared as a electrochemical sensing interface for detection of two volatile biomarkers, cyclic voltammetry studies showed that the fabricated sensor could detect 3-octanone in the range of 0~0.0025% (v/v) and with a detection limitation of 0.3 ppb, could detect butanone in the range of 0 ~ 0.055% (v/v), and with a detection limitation of 0.5 ppb, and exhibited good selectivity. The novel electrochemical biosensor combined with volatile biomarkers of gastric cancer owns great potential in applications such as early diagnosis and the prognosis of gastric cancer in near future.
机译：成功开发用于胃癌细胞的挥发性生物标记物超灵敏检测的新型电化学生物传感界面是一项艰巨的任务。在本文中，我们报道了筛选出与胃癌细胞相关的新型挥发性生物标志物，并开发了一种新型Au-Ag合金复合材料涂层的MWCNTs作为传感接​​口，用于超灵敏地检测挥发性生物标志物。在无血清培养基中培养MGC-803胃癌细胞和GES-1胃黏液细胞。优化了样品制备方法和HS-SPME条件，以筛选挥发性生物标志物。使用GC-MS鉴定了从细胞/培养基的顶空释放出的挥发物。制备了Au-Ag纳米颗粒涂层的多壁碳纳米管，作为检测挥发性生物标志物的传感界面。结果显示，在MGC-803细胞和GES-1细胞之间筛选出8种不同的挥发性代谢物。 MGC-803细胞的顶部空间中特别存在两种化合物，例如3-octanone和butanone。 MGC-803细胞和GES-1细胞的顶空共存3种挥发物，如4-异丙氧基丁醇，壬醇和4-丁氧基1-丁醇，其在GES-1细胞顶空的浓度明显高于在MGC-803细胞中，GES-1细胞的顶空仅存在三种挥发物，例如甲酸丙酯，1.4-丁二醇和2,6,11-三甲基十二烷。制备了负载金-银纳米颗粒的多壁碳纳米管的纳米复合材料，作为电化学传感界面，用于检测两种挥发性生物标志物，循环伏安法研究表明，所制备的传感器可以检测0〜0.0025％（v / v）范围内的3-辛酮。检出限为0.3 ppb，可以检测到0〜0.055％（v / v）范围内的丁酮，检出限为0.5 ppb，具有很好的选择性。结合胃癌挥发性生物标志物的新型电化学生物传感器在胃癌的早期诊断和预后等应用中具有巨大的潜力。

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