首页> 美国卫生研究院文献>Journal of Visualized Experiments : JoVE >Femoral Arterial and Venous Catheterization for Blood Sampling Drug Administration and Conscious Blood Pressure and Heart Rate Measurements
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Femoral Arterial and Venous Catheterization for Blood Sampling Drug Administration and Conscious Blood Pressure and Heart Rate Measurements

机译:股动脉和静脉导管插入术以进行血液采样药物管理以及有意识的血压和心率测量

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摘要

In multiple fields of study, access to the circulatory system in laboratory studies is necessary. Pharmacological studies in rats using chronically implanted catheters permit a researcher to effectively and humanely administer substances, perform repeated blood sampling and assists in conscious direct measurements of blood pressure and heart rate. Once the catheter is implanted long-term sampling is possible. Patency and catheter life depends on multiple factors including the lock solution used, flushing regimen and catheter material. This video will demonstrate the methodology of femoral artery and venous catheterization of the rat. In addition the video will demonstrate the use of the femoral venous and arterial catheters for blood sampling, drug administration and use of the arterial catheter in taking measurements of blood pressure and heart rate in a conscious freely-moving rat. A tether and harness attached to a swivel system will allow the animal to be housed and have samples taken by the researcher with minimal disruption to the animal. To maintain patency of the catheter, careful daily maintenance of the catheter is required using lock solution (100 U/ml heparinized saline), machine-ground blunt tip syringe needles and the use of syringe filters to minimize potential contamination. With careful aseptic surgical techniques, proper catheter materials and careful catheter maintenance techniques, it is possible to sustain patent catheters and healthy animals for long periods of time (several weeks).
机译:在多个研究领域中,有必要在实验室研究中使用循环系统。使用慢性植入导管对大鼠进行的药理研究使研究人员能够有效而人道地施用药物,进行重复的血液采样并协助有意识地直接测量血压和心率。植入导管后,便可以进行长期采样。通畅性和导管寿命取决于多种因素,包括使用的锁定溶液,冲洗方案和导管材料。该视频将演示大鼠股动脉和静脉置管的方法。此外,该视频还将演示使用股静脉和动脉导管进行血液采样,给药以及在有意识的自由运动大鼠中进行血压和心率测量时的使用。系在旋转系统上的系绳和安全带将允许饲养动物,并由研究人员在不破坏动物的情况下采集样本。为了保持导管的通畅,需要使用锁定溶液(100 U / ml肝素化的盐水),机器研磨的钝尖注射器针头以及使用注射器过滤器来仔细地每日维护导管,以最大程度地减少潜在的污染。通过仔细的无菌外科手术技术,适当的导管材料和仔细的导管维护技术,可以长时间维持专利导管和健康的动物(几周)。

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