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Paralytic Shellfish Toxin Uptake Assimilation Depuration and Transformation in the Southeast Asian Green-Lipped Mussel (Perna viridis)

机译:东南亚绿色唇贻贝(Perna viridis)中麻痹性贝类毒素的吸收吸收净化和转化

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摘要

Bivalve molluscs represent an important food source within the Philippines, but the health of seafood consumers is compromised through the accumulation of harmful algal toxins in edible shellfish tissues. In order to assess the dynamics of toxin risk in shellfish, this study investigated the uptake, depuration, assimilation, and analogue changes of paralytic shellfish toxins in Perna viridis. Tank experiments were conducted where mussels were fed with the toxic dinoflagellate Alexandrium minutum. Water and shellfish were sampled over a six day period to determine toxin concentrations in the shellfish meat and water, as well as algal cell densities. The maximum summed toxin concentration determined was 367 µg STX eq./100 g shellfish tissue, more than six times higher than the regulatory action limit in the Philippines. Several uptake and depuration cycles were observed during the study, with the first observed within the first 24 h coinciding with high algal cell densities. Toxin burdens were assessed within different parts of the shellfish tissue, with the highest levels quantified in the mantle during the first 18 h period but shifting towards the gut thereafter. A comparison of toxin profile data evidenced the conversion of GTX1,4 in the source algae to the less potent GTX2,3 in the shellfish tissue. Overall, the study illustrated the temporal variability in Perna viridis toxin concentrations during a modelled algal bloom event, and the accumulation of toxin from the water even after toxic algae were removed.
机译:双壳贝类软体动物是菲律宾的重要食物来源,但海产品消费者的健康却因食用贝类组织中有害藻类毒素的积累而受到损害。为了评估贝类毒素风险的动态变化,本研究调查了Perna viridis中麻痹性贝类毒素的摄取,净化,吸收和类似变化。进行坦克实验,在贻贝中喂食有毒的鞭毛藻亚历山大藻。在六天的时间内对水和贝类进行采样,以确定贝类肉和水中的毒素浓度以及藻类细胞密度。确定的最大总毒素浓度为367 µg STX当量/ 100 g贝类组织,比菲律宾的管制行动极限高出六倍以上。在研究过程中观察到了数个摄取和净化循环,其中第一个在最初的24小时内观察到藻类细胞密度高。在贝类组织的不同部位评估了毒素负荷,在头18小时内定量分析了披风中的最高含量,但此后向肠道转移。毒素谱数据的比较表明,源藻类中的GTX1,4转化为贝类组织中效力较弱的GTX2,3。总的来说,该研究说明了在模拟的藻华事件期间,天疱疮毒素浓度的时间变化,甚至去除了有毒藻类后,水中毒素的积累也是如此。

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