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首页> 美国卫生研究院文献>Toxins >Grape Seed Proanthocyanidin Extract Alleviates AflatoxinB1-Induced Immunotoxicity and Oxidative Stress via Modulation of NF-κB and Nrf2 Signaling Pathways in Broilers
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Grape Seed Proanthocyanidin Extract Alleviates AflatoxinB1-Induced Immunotoxicity and Oxidative Stress via Modulation of NF-κB and Nrf2 Signaling Pathways in Broilers

机译：葡萄籽原花青素提取物通过调节肉鸡中的NF-κB和Nrf2信号通路减轻黄曲霉毒素B1诱导的免疫毒性和氧化应激。

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Aflatoxin B1 (AFB1) is a widely spread mycotoxin contaminates food and feed, causing severe oxidative stress damages and immunotoxicity. Grape seed proanthocyanidin (GSPE), a natural antioxidant with wide range of pharmacological and medicinal properties. The goal of the present study was to investigate the protective effects of GSPE against AFB1-induced immunotoxicity and oxidative stress via NF-κB and Nrf2 signaling pathways in broiler chickens. For the experiment, 240 one-day old Cobb chicks were allocated into four dietary treatment groups of six replicates (10 birds per replicate): 1. Basal diet (control); 2. Basal diet + AFB1 1mg/kg contaminated corn (AFB1); 3. Basal diet + GSPE 250 mg/kg (GSPE); 4. Basal diet + AFB1 1 mg/kg + GSPE 250 mg/kg (AFB1 + GSPE). The results showed that GSPE significantly decreased serum inflammatory cytokines TNF-α, IFN-γ, IL-1β, IL-10, and IL-6 induced by AFB1. Similarly, GSPE + AFB1 treated group revealed a significant decrease in mRNA expressions of pro-inflammatory cytokines (TNF-α, IFN-γ, IL-1β, and IL-6) in the splenic tissue compared to the AFB1 treatment group. In addition, western blotting results manifested that GSPE treatment normalized the phosphorylation of nuclear factor kappa B (p65) and the degradation of IκBα protein induced by AFB1. Furthermore, GSPE enhanced the antioxidant defense system through activating the nuclear factor-erythroid-2-related factor (Nrf2) signaling pathway. The mRNA and protein expression level of Nrf2 and its down streaming associated genes were noted up-regulated by the addition of GSPE, and down-regulated in the AFB1 group. Taken together, GSPE alleviates AFB1-induced immunotoxicity and oxidative damage by inhibiting the NF-κB and activating the Nrf2 signaling pathways in broiler chickens. Conclusively, our results suggest that GSPE could be considered as a potential natural agent for the prevention of AFB1-induced immunotoxicity and oxidative damage.
机译：黄曲霉毒素B1（AFB1）是一种广泛传播的霉菌毒素，会污染食品和饲料，导致严重的氧化应激损害和免疫毒性。葡萄籽原花青素（GSPE），一种天然抗氧化剂，具有广泛的药理和药用特性。本研究的目的是研究GSPE通过NF-κB和Nrf2信号通路对AFB1诱导的免疫毒性和氧化应激的保护作用。对于该实验，将240只一天大的科布小鸡分成四个重复六个饮食的饮食治疗组（每个重复十只鸡）：1.基础饮食（对照组）； 2.基础饮食+ AFB1 1mg / kg受污染的玉米（AFB1）； 3.基础饮食+ GSPE 250 mg / kg（GSPE）； 4.基础饮食+ 1 mg / kg AFB1 + 250 mg / kg GSPE（AFB1 + GSPE）。结果表明，GSPE显着降低了AFB1诱导的血清炎性细胞因子TNF-α，IFN-γ，IL-1β，IL-10和IL-6。同样，与AFB1治疗组相比，GSPE + AFB1治疗组显示脾组织中促炎性细胞因子（TNF-α，IFN-γ，IL-1β和IL-6）的mRNA表达显着降低。此外，蛋白质印迹结果表明，GSPE处理使核因子κB（p65）的磷酸化和AFB1诱导的IκBα蛋白的降解正常化。此外，GSPE通过激活核因子-类胡萝卜素2相关因子（Nrf2）信号传导途径增强了抗氧化防御系统。 Nrf2的mRNA和蛋白表达水平及其下游相关基因通过添加GSPE被上调，而在AFB1组中被下调。总之，GSPE通过抑制肉鸡的NF-κB和激活Nrf2信号通路来减轻AFB1诱导的免疫毒性和氧化损伤。总之，我们的结果表明，GSPE可以被视为预防AFB1诱导的免疫毒性和氧化损伤的潜在天然药物。

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