首页> 美国卫生研究院文献>Journal of Zhejiang University. Science. B >Identification of Colletotrichum spp. isolated from strawberry in Zhejiang Province and Shanghai City China
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Identification of Colletotrichum spp. isolated from strawberry in Zhejiang Province and Shanghai City China

机译:鉴定炭疽菌。从中国浙江省和上海市的草莓中分离

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摘要

Strawberry anthracnose, caused by Colletotrichum spp., is a major disease of cultivated strawberry. This study identifies 31 isolates of Colletotrichum spp. which cause strawberry anthracnose in Zhejiang Province and Shanghai City, China. Eleven isolates were identified as C. acutatum, 10 as C. gloeosporioides and 10 as C. fragariae based on morphological characteristics, phylogenetic and sequence analyses. Species-specific polymerase chain reaction (PCR) and enzyme digestion further confirmed the identification of the Colletotrichum spp., demonstrating that these three species are currently the causal agents of strawberry anthracnose in the studied regions. Based on analysis of rDNA internal transcribed spacers (ITS) sequences, sequences of all C. acutatum were identical, and little genetic variability was observed between C. fragariae and C. gloeosporioides. However, the conservative nature of the MvnI specific site from isolates of C. gloeosporioides was confirmed, and this site could be used to differentiate C. gloeosporioides from C. fragariae.
机译:由炭疽菌引起的草莓炭疽病是栽培草莓的主要疾病。这项研究确定了炭疽菌的31种分离株。在浙江省和中国上海市区引起草莓炭疽病。根据形态特征,系统发育和序列分析,鉴定出11个分离物为硬壳梭菌,10个为球孢梭菌和10个为草莓角梭菌。物种特异性聚合酶链反应(PCR)和酶消化进一步证实了Colletotrichum spp。的鉴定,表明这三个物种目前是所研究地区草莓炭疽病的致病因子。基于对rDNA内部转录间隔区(ITS)序列的分析,所有cutcut acutatum的序列均相同,并且在草莓角衣壳菌和glossporioides之间的遗传变异很小。但是,证实了来自球孢梭菌分离物的MvnI特异性位点的保守性质,并且该位点可用于区分球孢梭菌和草莓角孢菌。

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