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首页> 美国卫生研究院文献>Journal of Zhejiang University. Science. B >Generation of insect-resistant and glyphosate-tolerant rice by introduction of a T-DNA containing two Bt insecticidal genes and an EPSPS gene
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Generation of insect-resistant and glyphosate-tolerant rice by introduction of a T-DNA containing two Bt insecticidal genes and an EPSPS gene

机译：通过导入含有两个Bt杀虫基因和EPSPS基因的T-DNA产生抗虫和耐草甘膦水稻

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Insect resistance and glyphosate tolerance have been two of the most important traits in the genetic improvement of various crops. In this study, two Bacillus thuringiensis (Bt) insecticidal genes, Cry1Ac and Cry1Ig, and a modified glyphosate-tolerant 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) gene (G10) were combined into a single transferred DNA (T-DNA) fragment and introduced into rice by Agrobacterium-mediated transformation. A transgenic line with single-copy T-DNA insertion named GAI-14 was found to be highly resistant to striped stem borer and rice leaf roller, and tolerant to glyphosate. Analysis of T-DNA border sequence suggested that the transgenes were inserted at the chromosome 3 and appeared to have not interrupted any known or putative genes. A field trial observed no significant difference in the basic agronomic traits between GAI-14 and the recipient rice.
机译：抗虫性和草甘膦耐受性已成为各种农作物遗传改良中最重要的两个特征。在这项研究中，两个苏云金芽孢杆菌（Bt）杀虫基因Cry1Ac和Cry1Ig，以及改良的草甘膦耐受性5-烯丙基丙酮酸shi草酸酯-3-磷酸合酶（EPSPS）基因（G10）被组合成单个转移的DNA（T-DNA）片段并通过农杆菌介导的转化导入水稻。发现具有单拷贝T-DNA插入的转基因品系GAI-14具有很高的抗条纹枯萎病和稻纵卷叶病的能力，并能耐受草甘膦。对T-DNA边界序列的分析表明，该转基因插入了3号染色体，并且似乎没有中断任何已知或推定的基因。田间试验发现，GAI-14和受精稻之间的基本农艺性状没有显着差异。

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