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首页> 美国卫生研究院文献>Virologica Sinica >Chevrier’s Field Mouse (Apodemus chevrieri) and Père David’s Vole (Eothenomys melanogaster) in China Carry Orthohepeviruses that form Two Putative Novel Genotypes Within the Species Orthohepevirus C
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Chevrier’s Field Mouse (Apodemus chevrieri) and Père David’s Vole (Eothenomys melanogaster) in China Carry Orthohepeviruses that form Two Putative Novel Genotypes Within the Species Orthohepevirus C

机译：Chevrier的田鼠（Apodemus chevrieri）和PèreDavid的Vole（Eothenomys melanogaster）在中国携带正痘病毒这些病毒在物种正痘病毒C中形成了两种推定的新基因型

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Hepatitis E virus (HEV) is the prototype of the family Hepeviridae and the causative agent of common acute viral hepatitis. Genetically diverse HEV-related viruses have been detected in a variety of mammals and some of them may have zoonotic potential. In this study, we tested 278 specimens collected from seven wild small mammal species in Yunnan province, China, for the presence and prevalence of orthohepevirus by broad-spectrum reverse transcription (RT)-PCR. HEV-related sequences were detected in two rodent species, including Chevrier’s field mouse (Apodemus chevrieri, family Muridae) and Père David’s vole (Eothenomys melanogaster, family Cricetidae), with the infection rates of 29.20% (59/202) and 7.27% (4/55), respectively. Further four representative full-length genomes were generated: two each from Chevrier’s field mouse (named RdHEVAc14 and RdHEVAc86) and Père David’s vole (RdHEVEm40 and RdHEVEm67). Phylogenetic analyses and pairwise distance comparisons of whole genome sequences and amino acid sequences of the gene coding regions showed that orthohepeviruses identified in Chinese Chevrier’s field mouse and Père David’s vole belonged to the species Orthohepevirus C but were highly divergent from the two assigned genotypes: HEV-C1 derived from rat and shrew, and HEV-C2 derived from ferret and possibly mink. Quantitative real-time RT-PCR demonstrated that these newly discovered orthohepeviruses had hepatic tropism. In summary, our work discovered two putative novel genotypes orthohepeviruses preliminarily named HEV-C3 and HEV-C4 within the species Orthohepevirus C, which expands our understanding of orthohepevirus infection in the order Rodentia and gives new insights into the origin, evolution, and host range of orthohepevirus.Electronic supplementary materialThe online version of this article (10.1007/s12250-018-0011-8) contains supplementary material, which is available to authorized users.
机译：戊型肝炎病毒（HEV）是肝炎病毒科的原型，也是常见的急性病毒性肝炎的病原体。已经在多种哺乳动物中发现了遗传多样性与HEV相关的病毒，其中一些可能具有人畜共患病的潜力。在这项研究中，我们通过广谱逆转录（RT）-PCR测试了从中国云南省的7个野生小型哺乳动物物种中收集的278个标本中正疱疹病毒的存在和流行。在两个啮齿动物物种中检测到了HEV相关序列，包括Chevrier的田鼠（Apodemus chevrieri，鼠科）和PèreDavid的田鼠（Eothenomys melanogaster，Cricetidae），感染率分别为29.20％（59/202）和7.27％（ 4/55）。此外，还生成了四个有代表性的全长基因组：分别来自Chevrier的田鼠（分别名为RdHEVAc14和RdHEVAc86）和PèreDavid的田鼠（RdHEVEm40和RdHEVEm67）两个。系统发育分析和基因编码区的全基因组序列及氨基酸序列的成对距离比较表明，在中国Chevrier田鼠和PèreDavid田鼠中鉴定出的正肝炎病毒属于Orthohepevirus C物种，但与两种指定的基因型HEV-高度不同C1来自老鼠和sh，HEV-C2来自雪貂和可能的貂。实时定量RT-PCR证明这些新发现的正肝病毒具有肝嗜性。总而言之，我们的工作在Orthohepevirus C物种中发现了两种推定的新基因型Orthohepeviruses，它们最初被命名为HEV-C3和HEV-C4，从而以啮齿动物的顺序扩展了我们对Orthohepevirus感染的了解，并提供了对起源，进化和宿主范围的新见解。电子补充材料本文的在线版本（10.1007 / s12250-018-0011-8）包含补充材料，可供授权用户使用。

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