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首页> 美国卫生研究院文献>Journal of Zhejiang University. Science. B >Antagonistic interaction between Trichoderma asperellum and Phytophthora capsici in vitro
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Antagonistic interaction between Trichoderma asperellum and Phytophthora capsici in vitro

机译：曲霉木霉与辣椒疫霉菌的体外拮抗作用

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摘要

Phytophthora capsici is a phytopathogen that causes a destructive pepper blight that is extremely difficult to control. Using a fungicide application against the disease is costly and relatively ineffective and there is also a huge environmental concern about the use of such chemicals. The genus Trichoderma has been known to have a potential biocontrol issue. In this paper we investigate the mechanism for causing the infection of T. asperellum against P. capsici. Trichoderma sp. (isolate CGMCC 6422) was developed to have a strong antagonistic action against hyphae of P. capsici through screening tests. The strain was identified as T. asperellum through using a combination of morphological characteristics and molecular data. T. asperellum was able to collapse the mycelium of the colonies of the pathogen through dual culture tests by breaking down the pathogenic hyphae into fragments. The scanning electron microscope showed that the hyphae of T. asperellum surrounded and penetrated the pathogens hyphae, resulting in hyphal collapse. The results show that seven days after inoculation, the hyphae of the pathogen were completely degraded in a dual culture. T. asperellum was also able to enter the P. capsici oospores through using oogonia and then developed hyphae and produced conidia, leading to the disintegration of the oogonia and oospores. Seven days after inoculation, an average 10.8% of the oospores were infected, but at this stage, the structures of oospores were still intact. Subsequently, the number of infected oospores increased and the oospores started to collapse. Forty-two days after inoculation, almost all the oospores were infected, with 9.3% of the structures of the oospores being intact and 90.7% of the oospores having collapsed.
机译：辣椒疫霉是一种植物病原体，会引起极度难以控制的破坏性辣椒枯萎病。使用杀真菌剂来对抗该疾病是昂贵的并且相对无效的，并且对于使用此类化学品还存在巨大的环境关注。木霉属已知具有潜在的生物防治问题。在本文中，我们研究了引起辣椒毛霉菌感染辣椒的机制。木霉属通过筛选试验，发现（分离的CGMCC 6422）对辣椒辣椒的菌丝具有强的拮抗作用。通过结合形态学特征和分子数据将菌株鉴定为曲霉。曲霉菌通过将病原菌丝分解成碎片，通过双重培养试验能够破坏病原菌菌落的菌丝体。扫描电子显微镜显示，曲霉菌丝的菌丝包围并穿透病原菌的菌丝，导致菌丝塌陷。结果表明，接种后7天，病原菌的菌丝在双重培养中被完全降解。 T. asperellum也能够通过使用卵菌进入小球藻卵菌，然后形成菌丝并产生分生孢子，从而导致卵菌和卵孢子分解。接种后7天，平均有10.8％的卵菌被感染，但是在这个阶段，卵菌的结构仍然完好无损。随后，被感染的卵孢子的数量增加，卵孢子开始崩溃。接种后四十二天，几乎所有的卵子都被感染，卵子的9.3％的结构完好无损，卵子的90.7％已塌陷。

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