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Chryseobacterium chengduensis sp. nov. isolated from the air of captive giant panda enclosures in Chengdu China

机译:成都氏杆菌十一月与中国成都的大熊猫圈养圈隔离

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摘要

A Gram-negative, aerobic, non-motile, rod-shaped bacterial strain, designated 25-1T, was isolated from the air inside giant panda enclosures at the Chengdu Research Base of Giant Panda Breeding, China. Strain 25-1T grew optimally at pH 7.0–8.0, at 28–30 °C and in the presence of NaCl concentrations from 0.0% to 0.5 %. 16S rRNA gene sequence analysis indicated that strain 25-1T belongs to the genus Chryseobacterium within the family Flavobacteriaceae and is related most closely to C. carnis G81T (96.4% similarity), C. lathyri RBA2-6T (95.8% similarity), and C. zeae JM1085T (95.8% similarity). Its genomic DNA G+C molar composition was 36.2%. The major cellular fatty acids were iso-C15:0 (44.0%), iso-C17:0 3OH (19.8%) and C16:1 ω7c/16:1 ω6c (12.7%). The only isoprenoid quinone was menaquinone 6 (MK-6). The major polar lipids were phosphatidylethanolamine, two unidentified amino lipids and two unidentified lipids. The DNA–DNA relatedness between strain 25-1T and C. lathyri RBA2-6T was 38%. Phenotypic, genotypic, and phylogenetic characteristics indicated that strain 25-1T is a novel member of the genus Chryseobacterium, for which the name C. chengduensis sp. nov. is proposed. The type strain is 25-1T (CCTCC AB2015133T=DSM 100396T).
机译:在中国大熊猫繁育成都研究基地的大熊猫圈舍内,从空气中分离出一种革兰氏阴性,需氧,不能运动的杆状细菌菌株,称为25-1 T 。 。 25-1 T 菌株在pH 7.0–8.0、28–30°C和NaCl浓度为0.0%至0.5%的条件下最佳生长。 16S rRNA基因序列分析表明,菌株25-1 T 属于黄杆菌科的金黄色葡萄球菌,与C. carnis G81 T 关系最密切(相似性为96.4%) ),C。lathyri RBA2-6 T (相似度为95.8%)和C. zeae JM1085 T (相似度为95.8%)。其基因组DNA G + C摩尔组成为36.2%。主要的细胞脂肪酸为iso-C15:0(44.0%),iso-C17:0 3OH(19.8%)和C16:1ω7c/ 16:1ω6c(12.7%)。唯一的类异戊二烯醌是甲萘醌6(MK-6)。主要的极性脂质是磷脂酰乙醇胺,两个未鉴定的氨基脂质和两个未鉴定的脂质。菌株25-1 T 和Lathyri RBA2-6 T 之间的DNA-DNA相关性为38%。表型,基因型和系统发生特征表明,菌株25-1 T 是金黄色葡萄球菌属的一个新成员,命名为C. chengduensis sp。十一月被提议。类型株为25-1 T (CCTCC AB2015133 T = DSM 100396 T )。

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