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Mycoviral Population Dynamics in Spanish Isolates of the Entomopathogenic Fungus Beauveria bassiana

机译：西班牙病原性真菌球孢白僵菌分离株中的分枝杆菌病毒种群动态。

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The use of mycoviruses to manipulate the virulence of entomopathogenic fungi employed as biocontrol agents may lead to the development of novel methods to control attacks by insect pests. Such approaches are urgently required, as existing agrochemicals are being withdrawn from the market due to environmental and health concerns. The aim of this work is to investigate the presence and diversity of mycoviruses in large panels of entomopathogenic fungi, mostly from Spain and Denmark. In total, 151 isolates belonging to the genera Beauveria, Metarhizium, Lecanicillium, Purpureocillium, Isaria, and Paecilomyces were screened for the presence of dsRNA elements and 12 Spanish B. bassiana isolates were found to harbor mycoviruses. All identified mycoviruses belong to three previously characterised species, the officially recognised Beauveria bassiana victorivirus 1 (BbVV-1) and the proposed Beauveria bassiana partitivirus 2 (BbPV-2) and Beauveria bassiana polymycovirus 1 (BbPmV-1); individual B. bassiana isolates may harbor up to three of these mycoviruses. Notably, these mycovirus species are under distinct selection pressures, while recombination of viral genomes increases population diversity. Phylogenetic analysis of the RNA-dependent RNA polymerase gene sequences revealed that the current population structure in Spain is potentially a result of both vertical and horizontal mycovirus transmission. Finally, pathogenicity experiments using the Mediterranean fruit fly Ceratitis capitata showed no direct correlation between the presence of any particular mycovirus and the virulence of the B. bassiana isolates, but illustrated potentially interesting isolates that exhibit relatively high virulence, which will be used in more detailed virulence experimentation in the future.
机译：使用分枝杆菌病毒来操纵用作生物防治剂的昆虫病原真菌的毒性可能会导致开发控制虫害攻击的新方法。迫切需要这种方法，因为由于环境和健康方面的考虑，现有的农用化学品已从市场上撤出。这项工作的目的是调查大部分西班牙和丹麦的昆虫病原真菌中真菌病毒的存在和多样性。总共筛选了属于白僵菌属，金属属，Lecanicillium，purpureocillium，Isaria和Paecilomyces属的151种分离株，以检测是否存在dsRNA元件，并发现了12个西班牙球茎巴斯德氏菌分离株带有分枝杆菌病毒。所有已鉴定的分枝杆菌病毒均属于三个先前鉴定的物种，即官方认可的球孢白僵菌维多利亚病毒1（BbVV-1）和拟议的球孢白僵菌partitivirus 2（BbPV-2）和球孢白僵菌多粘病毒1（BbPmV-1）；单独的球孢杆菌分离物最多可以包含三种霉菌病毒。值得注意的是，这些分枝杆菌病毒在不同的选择压力下，而病毒基因组的重组增加了种群多样性。对RNA依赖性RNA聚合酶基因序列的系统进化分析表明，西班牙目前的种群结构可能是垂直和水平霉菌病毒传播的结果。最后，使用地中海果蝇ceratitis capitata进行的致病性实验显示，任何特定的分枝杆菌病毒的存在与球孢杆菌分离株的毒力之间都没有直接关联，但说明了潜在有趣的分离株表现出较高的毒力，将在更详细的说明中使用未来的毒力实验。

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