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首页> 美国卫生研究院文献>World Journal of Gastroenterology >Effect of notoginsenoside R1 on hepatic microcirculation disturbance induced by gut ischemia and reperfusion
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Effect of notoginsenoside R1 on hepatic microcirculation disturbance induced by gut ischemia and reperfusion

机译：三七皂苷R1对肠缺血再灌注肝微循环障碍的影响

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AIM: To assess the effect of notoginsenoside R1 on hepatic microcirculatory disturbance induced by gut ischemia/reperfusion (I/R) in mice.METHODS: The superior mesenteric artery (SMA) of C57/BL mice was ligated for 15 min to induce gut ischemia followed by 30-min reperfusion. In another set of experiments, R1 was continuously infused (10 mg/kg per hour) from 10 min before I/R until the end of the investigation to study the influence of R1 on hepatic microcirculatory disturbance induced by gut I/R. Hepatic microcirculation was observed by inverted microscopy, and the vascular diameter, red blood cell (RBC) velocity and sinusoid perfusion were estimated. Leukocyte rolling and adhesion were observed under a laser confocal microscope. Thirty and 60 min after reperfusion, lactate dehydrogenase (LDH), alanine aminotransferase (ALT) and aspartate transaminase (AST) in peripheral blood were determined. The expression of adhesion molecules CD11b/CD18 in neutrophils and tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6) and monocyte chemotactic protein-1 (MCP-1) in plasma were evaluated by flow cytometry. E-selectin and intercellular adhesion molecule-1 (ICAM-1) in hepatic tissue were examined by immunofluorescence.RESULTS: After gut I/R, the diameters of terminal portal venules and central veins, RBC velocity and the number of perfused sinusoids were decreased, while the leukocyte rolling and adhesion, the expression of E-selectin in hepatic vessels and CD18 in neutrophils, IL-6, MCP-1, LDH, ALT and AST were increased. R1 treatment attenuated these alterations except for IL-6 and MCP-1.CONCLUSION: R1 prevents I/R-induced hepatic microcirculation disturbance and hepatocyte injury. The effect of R1 is related to its inhibition of leukocyte rolling and adhesion by inhibiting the expression of E-selectin in endothelium and CD18 in neutrophils.
机译：目的：评价三七皂苷R1对小鼠肠缺血再灌注（I / R）引起的肝微循环障碍的作用。方法：结扎C57 / BL小鼠肠系膜上动脉（SMA）15分钟，诱导肠缺血然后再灌注30分钟。在另一组实验中，从I / R前10分钟到研究结束，持续注入R1（每小时10 mg / kg），以研究R1对肠道I / R引起的肝微循环障碍的影响。倒置显微镜观察肝微循环，并评估血管直径，红细胞（RBC）速度和正弦曲线灌注。在激光共聚焦显微镜下观察白细胞滚动和粘附。再灌注后30和60分钟，测定外周血中的乳酸脱氢酶（LDH），丙氨酸转氨酶（ALT）和天冬氨酸转氨酶（AST）。通过流式细胞术评估血浆中粘附分子CD11b / CD18和血浆中肿瘤坏死因子-α（TNF-α），白细胞介素6（IL-6）和单核细胞趋化蛋白-1（MCP-1）的表达。结果：肠道I / R后，肠道门静脉和中央静脉的直径，RBC速度和灌注的正弦波数量均减少，肝组织中的E-选择素和细胞间粘附分子-1（ICAM-1）得以检测。 ，在白细胞滚动和粘附的同时，中性粒细胞，IL-6，MCP-1，LDH，ALT和AST的肝血管中E-选择蛋白的表达和CD18的表达增加。除IL-6和MCP-1外，R1治疗可减轻这些改变。结论：R1可预防I / R引起的肝微循环障碍和肝细胞损伤。 R1的作用与其通过抑制嗜中性粒细胞的内皮细胞E-选择素和CD18的表达抑制白细胞滚动和粘附有关。

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