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首页> 美国卫生研究院文献>World Journal of Gastroenterology >Studied microRNA gene expression in human hepatocellular carcinoma by microRNA microarray techniques
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Studied microRNA gene expression in human hepatocellular carcinoma by microRNA microarray techniques

机译：应用microRNA芯片技术研究人肝细胞癌中microRNA基因的表达

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AIM: To achieve a better understanding of the molecular mechanisms of microRNA expression changes involved in hepatocellular carcinoma.METHODS: In this research process, patients were not treated with antivirals, immunosuppressants or immunomodulators for at least 6 mo before collecting serum. The study population was composed of 35 outpatient hepatitis B virus (HBV) cases and 12 healthy control cases from the Affiliated Hospital of Inner Mongolia Medical University (Inner Mongolia, China) from July 2013 to April 2014. The 35 HBV cases were divided into two groups: a hepatocirrhosis group with 20 cases and a liver cancer group with 15 cases. All 35 cases carried HBsAg. The diagnostic criteria followed the European Association for the Study of the Liver 2012 (EASL2012) standards. MicroRNA (miRNA) was extracted from a control group of patients, a group with hepatocirrhosis and a group with liver cancer and its quality was analyzed using the human V2 microRNA expression beadchip. Cluster analysis and a radar chart were then applied to the miRNA changes.RESULTS: The miRNA-qualified rate of human serum samples was 93%. The concentration of a single sample was > 200 ng/μL and the volume was > 5 μL. All miRNA serum samples were uncontaminated by the genome. The Mann-Whitney test showed significant differences in miRNA between each group, with a detection P-value of < 0.05. Illumina software was set up with Diff Score set to ± 13, meaning that P = 0.001.There were significant changes in miRNA expression between the three groups. miRNA-183 was the most up-regulated, followed by miRNA-373. miRNA-129 and miRNA-188 were both strongly down-regulated and miRNA-378 was down-regulated a small amount. The liver cancer group had greater changes, which indicated that changes in miRNA expression levels were caused by hepatocirrhosis. The liver cancer disease course then further increased these changes. In the pentagon created by these five miRNAs, three groups showed significant deviation. The liver cancer group had a bigger deviation trend. The chart indicated that miRNA expression changes occurred in the hepatocirrhosis group, which increased in the liver cancer disease course and were irreversible.CONCLUSION: There was a significant relationship between the irreversible up-regulation of miRNA-183/373 and down-regulation of miRNA-129/188/378 and incidences of hepatocirrhosis and liver cancer.
机译：目的：为了更好地了解参与肝细胞癌的微小RNA表达变化的分子机制。方法：在该研究过程中，患者在收集血清之前未接受抗病毒药，免疫抑制剂或免疫调节剂至少6个月的治疗。研究人群由2013年7月至2014年4月内蒙古医科大学附属医院（中国内蒙古）的35例门诊乙型肝炎病毒（HBV）病例和12例健康对照病例组成。这35例HBV病例分为两部分分组：肝硬化组20例，肝癌组15例。 35例均携带HBsAg。诊断标准遵循欧洲肝脏研究协会（EASL2012）标准。从患者的对照组，肝硬化组和肝癌组中提取MicroRNA（miRNA），并使用人类V2 microRNA表达珠芯片分析其质量。结果：miRNA检定率为93％。单个样品的浓度> 200 ng /μL，体积> 5μL。所有miRNA血清样品均未受到基因组污染。 Mann-Whitney测试显示，每组之间的miRNA差异显着，检测P值<0.05。将Illumina软件的Diff分数设置为±13，这意味着P = 0.001。三组之间的miRNA表达有显着变化。 miRNA-183上调最多，其次是miRNA-373。 miRNA-129和miRNA-188均被强烈下调，miRNA-378被少量下调。肝癌组的变化更大，这表明miRNA表达水平的变化是由肝硬化引起的。然后，肝癌疾病进程进一步增加了这些变化。在这五个miRNA产生的五边形中，三组显示出明显的偏差。肝癌组有较大的偏差趋势。该图表明，肝硬化组miRNA表达发生变化，在肝癌病程中增加且不可逆。结论：miRNA-183 / 373的不可逆上调与miRNA的下调之间存在显着相关性-129/188/378和肝硬化和肝癌的发生率。

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