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Data supporting the rat brain sample preparation and validation assays for simultaneous determination of 8 neurotransmitters and their metabolites using liquid chromatography–tandem mass spectrometry

机译:使用液相色谱-串联质谱法同时测定8种神经递质及其代谢物的大鼠脑样本制备和验证测定的数据

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摘要

The data presented in this article supports the rat brain sample preparation procedure previous to its injection into the liquid chromatography–tandem mass spectrometry (LC–MS/MS) system to monitor levels of adrenaline, noradrenaline, glutamic acid, γ-aminobutyric acid, dopamine, 5-hydroxytryptamine, 5-hydroxyindole acetic acid, and 3-methoxy-4-hydroxyphenylglycol. In addition, we describe the method validation assays (such as calibration curve, lower limit of quantification, precision and accuracy intra- and inter-day, selectivity, extraction recovery and matrix effect, stability, and carry-over effect) according to the United States Food and Drug Administration and European Medicine Agency to measure in one step different neurotransmitters and their metabolites. The data supplied in this article is related to the research study entitled: “Simultaneous determination of 8 neurotransmitters and their metabolite levels in rat brain using liquid chromatography in tandem with mass spectrometry: application to the murine Nrf2 model of depression” (Wojnicz et al. 2016) .
机译:本文提供的数据支持将大鼠脑样品注射到液相色谱-串联质谱(LC-MS / MS)系统中之前的程序,以监测肾上腺素,去甲肾上腺素,谷氨酸,γ-氨基丁酸,多巴胺的水平,5-羟基色胺,5-羟基吲哚乙酸和3-甲氧基-4-羟基苯基乙二醇。此外,我们还描述了根据United States进行的方法验证测定(例如校准曲线,定量下限,日内和日间精密度和准确性,选择性,萃取回收率和基质效应,稳定性和残留效应)。美国食品药品监督管理局和欧洲医学局一步一步地测量不同的神经递质及其代谢产物。本文提供的数据与以下研究相关:``液相色谱-质谱联用同时测定大鼠脑中8种神经递质及其代谢物的含量:在鼠Nrf2抑郁模型中的应用''(Wojnicz等。 2016)。

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