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Performance comparison of four commercially available cytometers using fluorescent polystyrene submicron-scale beads

机译:使用荧光聚苯乙烯亚微米级微珠的四种市售细胞仪的性能比较

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摘要

Accurate comparison of flow cytometric data requires an understanding of how the cytometric fingerprint of a sample may vary from instrument to instrument. Key sources of variability include the number, wavelengths, and power of excitation lasers; the number and types of emission detectors; sample-handling systems and options; and whether fixed or dynamic detector voltages are used. To explore this variability, suspensions of three sizes (0.2, 0.5, and 0.8 μm-diameter) of solid, fluorescent, polystyrene beads were prepared. The suspensions were then run on four flow cytometers, keeping instrument settings as consistent as possible. The results are displayed graphically in Figure 3 of the article “Flow cytometry applications in water treatment, distribution, and reuse: A review” (DOI: ) [1]. This dataset contains the complete FCS files generated from the experimental comparison. In the development and application of flow cytometry to water quality assessment, we recommend data sharing in this manner to enable comprehensive reporting, meaningful comparison of results obtained using different cytometer models, enhanced exploration of data along multiple parameters, and use of acquired data for computational advancements in the field.
机译:准确比较流式细胞仪数据需要了解样品的细胞仪指纹在不同仪器之间如何变化。可变性的主要来源包括激发激光器的数量,波长和功率;排放探测器的数量和类型;样品处理系统和选项;以及是否使用固定或动态检测器电压。为了探索这种可变性,制备了三种尺寸(直径为0.2、0.5和0.8μm)的固体荧光聚苯乙烯珠的悬浮液。然后将悬浮液在四个流式细胞仪上运行,并保持仪器设置尽可能一致。结果以图形方式显示在文章“流式细胞术在水处理,分配和再利用中的应用:评论”(DOI:)[3]中。该数据集包含根据实验比较生成的完整FCS文件。在流式细胞术在水质评估中的开发和应用中,我们建议以这种方式进行数据共享,以实现全面报告,使用不同细胞仪模型获得的结果进行有意义的比较,沿多个参数加强对数据的探索以及将获得的数据用于计算该领域的进步。

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