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首页> 美国卫生研究院文献>Current Developments in Nutrition >Ingesting a Combined Carbohydrate and Essential Amino Acid Supplement Compared to a Non-Nutritive Placebo Blunts Mitochondrial Biogenesis-Related Gene Expression after Aerobic Exercise
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Ingesting a Combined Carbohydrate and Essential Amino Acid Supplement Compared to a Non-Nutritive Placebo Blunts Mitochondrial Biogenesis-Related Gene Expression after Aerobic Exercise

机译：与非营养性安慰剂相比摄入碳水化合物和必需氨基酸补充剂能使有氧运动后的线粒体生物发生相关基因表达降低。

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>Background: Whether load carriage (LC), an endurance exercise mode composed of the aerobic component of traditional endurance exercise [e.g., cycle ergometry (CE)] and contractile forces characteristic of resistive-type exercise, modulates acute mitochondrial adaptive responses to endurance exercise and supplemental nutrition [carbohydrate + essential amino acids (CHO+EAA)] is not known. >Objective: The aim of this study was to examine the effects of LC and CE, with or without CHO+EAA supplementation, on acute markers of mitochondrial biogenesis. >Methods: Twenty-five adults performed 90 min of metabolically matched LC (treadmill walking, wearing a vest equal to 30% of body mass) or CE exercise during which CHO+EAA (46 g carbohydrate and 10 g essential amino acids) or non-nutritive control (CON) drinks were consumed. Muscle biopsy samples were collected at rest (pre-exercise), post-exercise, and after 3 h of recovery to assess citrate synthase activity and the expression of mRNA (reverse transcriptase–quantitative polymerase chain reaction) and protein (Western blot). >Results: Citrate synthase and phosphorylated p38 mitogen-activated protein kinase (p38 MAPK)Thr180/Tyr182 were elevated postexercise compared with pre-exercise (time main effect, P < 0.05). Peroxisome proliferator-activated γ-receptor coactivator 1α (PGC-1α) expression was highest after recovery for CE compared with LC (exercise-by-time effect, P < 0.05). Sirtuin 1 (SIRT1) expression postexercise was higher for CON than for CHO+EAA treatments (drink-by-time, P < 0.05). Tumor suppressor p53 (p53), mitochondrial transcription factor A (TFAM), and cytochrome c oxidase subunit IV (COXIV) expression was greater for CON than for CHO+EAA treatments (drink main effect, P < 0.05). PGC-1α and p53 expressions were positively associated (P < 0.05) with TFAM (r = 0.629 and 0.736, respectively) and COXIV (r = 0.465 and 0.461, respectively) expressions. >Conclusions: Acute mitochondrial adaptive responses to endurance exercise appear to be largely driven by exogenous nutrition availability. Although CE upregulated PGC-1α expression to a greater extent than LC, downstream signaling was the same between modes, suggesting that LC, in large part, elicits the same acute mitochondrial response as traditional, non–weight-bearing endurance exercise. This trial was registered at clinicaltrials.gov as .
机译：>背景：负荷运输（LC）是一种由传统耐力运动的有氧成分[例如，循环测功（CE）]和阻力型运动特有的收缩力组成的耐力运动模式，是否可以调节急性线粒体对耐力运动和补充营养[碳水化合物+必需氨基酸（CHO + EAA）]的适应性反应尚不清楚。 >目的：该研究的目的是研究LC和CE（添加或不添加CHO + EAA）对线粒体生物发生急性标志物的影响。 >方法： 25名成年人进行了90分钟的代谢匹配LC（跑步机行走，穿着相当于体重的30％的背心）或进行CE + EAA（46 g碳水化合物和10 g碳水化合物）的CE运动食用必需氨基酸）或非营养性对照（CON）饮料。在静止（运动前），运动后和恢复3小时后收集肌肉活检样本，以评估柠檬酸合酶活性以及mRNA（逆转录酶-定量聚合酶链反应）和蛋白质的表达（蛋白质印迹）。 >结果：与运动前相比，运动后柠檬酸合酶和磷酸化的p38丝裂原活化蛋白激酶（p38 MAPK） Thr180 / Tyr182 升高（时间主效应，P <0.05） 。 CE恢复后，过氧化物酶体增殖物激活的γ受体共激活因子1α（PGC-1α）的表达高于LC（随时间变化的运动效果，P <0.05）。 CON后运动后Sirtuin 1（SIRT1）的表达高于CHO + EAA治疗（按时间饮用，P <0.05）。 CON的肿瘤抑制因子p53（p53），线粒体转录因子A（TFAM）和细胞色素C氧化酶亚基IV（COXIV）的表达要比CHO + EAA处理的要大（饮料主要作用，P <0.05）。 PGC-1α和p53表达与TFAM（分别为r = 0.629和0.736）和COXIV（分别为r = 0.465和0.461）表达正相关（P <0.05）。 >结论：对耐力运动的急性线粒体适应性反应似乎主要是由外源营养的可获得性驱动的。尽管CE上调了PGC-1α的表达，但其在下游的信号传导方式与LC相同，这表明LC在很大程度上引起了与传统的非线粒体相同的急性线粒体反应。耐力运动。该试验已在Clinicaltrials.gov上注册为。

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