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首页> 美国卫生研究院文献>Current Chemical Genomics and Translational Medicine >A Comparison of the Performance and Application Differences Between Manual and Automated Patch-Clamp Techniques
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A Comparison of the Performance and Application Differences Between Manual and Automated Patch-Clamp Techniques

机译：手动和自动膜片钳技术的性能和应用差异的比较

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摘要

The patch clamp technique is commonly used in electrophysiological experiments and offers direct insight into ion channel properties through the characterization of ion channel activity. This technique can be used to elucidate the interaction between a drug and a specific ion channel at different conformational states to understand the ion channel modulators’ mechanisms. The patch clamp technique is regarded as a gold standard for ion channel research; however, it suffers from low throughput and high personnel costs. In the last decade, the development of several automated electrophysiology platforms has greatly increased the screen throughput of whole cell electrophysiological recordings. New advancements in the automated patch clamp systems have aimed to provide high data quality, high content, and high throughput. However, due to the limitations noted above, automated patch clamp systems are not capable of replacing manual patch clamp systems in ion channel research. While automated patch clamp systems are useful for screening large amounts of compounds in cell lines that stably express high levels of ion channels, the manual patch clamp technique is still necessary for studying ion channel properties in some research areas and for specific cell types, including primary cells that have mixed cell types and differentiated cells that derive from induced pluripotent stem cells (iPSCs) or embryonic stem cells (ESCs). Therefore, further improvements in flexibility with regard to cell types and data quality will broaden the applications of the automated patch clamp systems in both academia and industry.
机译：膜片钳技术通常用于电生理实验，并通过表征离子通道活性提供对离子通道特性的直接了解。该技术可用于阐明药物与特定构象态下特定离子通道之间的相互作用，以了解离子通道调节剂的机制。膜片钳技术被认为是离子通道研究的金标准。但是，它具有产量低和人员成本高的缺点。在过去的十年中，几个自动电生理平台的发展大大提高了全细胞电生理记录的屏幕通量。自动膜片钳系统的新进展旨在提供高数据质量，高内容和高吞吐量。但是，由于上述限制，在离子通道研究中，自动膜片钳系统无法替代手动膜片钳系统。尽管自动膜片钳系统可用于筛选稳定表达高水平离子通道的细胞系中的大量化合物，但对于某些研究领域和特定细胞类型（包括原代）的离子通道特性研究，仍然需要手动膜片钳技术具有混合细胞类型的细胞和源自诱导多能干细胞（iPSC）或胚胎干细胞（ESC）的分化细胞。因此，关于细胞类型和数据质量的灵活性的进一步提高将扩大自动膜片钳系统在学术界和工业界的应用。

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