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首页> 美国卫生研究院文献>Comparative Medicine >P26-based Serodiagnosis for Bartonella spp. Infection in Cats
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P26-based Serodiagnosis for Bartonella spp. Infection in Cats

机译：基于P26的Bartonella spp血清学诊断。猫感染

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Bartonella henselae P26 has been identified as an immunodominant antigen expressed during feline infection. We used antisera from cats experimentally infected with B. henselae (n = 6), B. clarridgeiae (n = 4), or B. koehlerae (n = 2) and from a sample of naturally infected cats (B. henselae, n = 34; B. clarridgeiae, n = 1) to evaluate recombinant P26 (rP26) as a serodiagnostic antigen. Immunoblots using antisera from cats infected with B. henselae and B. clarridgeiae reacted strongly with rP26, whereas B. koehlerae antisera did not. A capture ELISA was designed to evaluate the kinetics of rP26 IgG in sera from experimentally infected cats. For B. henselae and B. clarridgeiae antisera, the kinetic profiles of reactivity were similar for rP26 capture ELISA and Bartonella spp. indirect fluorescence assay. However, for B. koehlerae antisera, reactivity in rP26 capture ELISA was consistently low. The serodiagnostic potential of rP26 capture ELISA was evaluated using sera from cats with known Bartonella sp. exposure histories. All 24 (100%) uninfected cats were seronegative, and 33 of 35 (94.3%) cats bacteremic for Bartonella spp. were seropositive. We propose that rP26-based serology can serve as a useful adjunct tool for the diagnosis of feline infection with B. henselae and B. clarridgeiae, but it may not be useful for feline infection with B. koehlerae.
机译：汉赛巴尔通体P26已被鉴定为猫感染期间表达的免疫优势抗原。我们使用了经过实验感染过汉森双歧杆菌（n = 6），克拉克杆菌clarridgeiae（n = 4）或B. koehlerae（n = 2）的猫的抗血清，以及从自然感染猫的样本（hen。B. henselae，n = 34；克拉氏芽孢杆菌，n ＝ 1）以评估重组P26（rP26）作为血清诊断抗原。使用抗血清的免疫印迹法从感染了亨氏杆菌和克拉氏芽孢杆菌的猫中与rP26强烈反应，而koehlerae血清则没有。设计了捕获ELISA，以评估来自实验感染猫的血清中rP26 IgG的动力学。对于亨氏芽孢杆菌和克拉丽芽孢杆菌抗血清，反应性动力学图谱与rP26捕获ELISA和Bartonella spp相似。间接荧光测定。但是，对于小芽孢杆菌抗血清，rP26捕获ELISA中的反应性始终很低。 rP26捕获ELISA的血清诊断潜力使用已知Bartonella sp。的猫的血清进行评估。接触历史。所有24只（100％）未感染的猫都是血清阴性的，而35只猫（94.3％）中的33只对巴通体属菌具有细菌性。呈血清阳性。我们建议基于rP26的血清学可以作为诊断B. henselae和 B的猫感染的有用辅助工具。 clarridgeiae ，但对于猫用 B感染可能没有用。 koehlerae 。

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