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首页> 美国卫生研究院文献>Clinical and Experimental Immunology >Localization of intrinsic factor and complement fixing intrinsic factor–intrinsic factor antibody complex in parietal cell of man
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Localization of intrinsic factor and complement fixing intrinsic factor–intrinsic factor antibody complex in parietal cell of man

机译：人顶壁细胞中内在因子和补体固定内在因子-内在因子抗体复合物的定位

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In an attempt to localize intrinsic factor in the human parietal cell, and to study its intracellular union with the intrinsic factor antibody and complement, intrinsic factor antibody was separated from coexisting parietal cell antibody in pernicious anaemia sera by gel filtration. Intrinsic factor antibody of both `binding' and `blocking' type was also produced in rabbits by immunization with semi-purified human intrinsic factor–[57Co]B12 complex.Intrinsic factor antibody obtained from both sources produced fluorescence in the human parietal cells in the indirect Coons' test in the presence of fluoresceinated anti-human IgG. The fluorescence was localized peripherally, at the cell membrane. When instead of the fluoresceinated anti-human IgG a fluoresceinated anti-human complement (C) serum and normal complement containing serum were used, intrinsic factor antibody from both sources produced fluorescence of the entire parietal cell cytoplasm of the human mucosa.Thus, intrinsic factor was localized at highest concentration at the membrane of the parietal cell in man, the intrinsic factor antibody–intrinsic factor complex was demonstrated within the human parietal cell, and evidence was obtained that this antigen–antibody complex fixes complement (C). The possible role of the intrinsic factor–intrinsic factor antibody–complement complex in the development of gastric atrophy in pernicious anaemia has been considered.
机译：为了将固有因子定位在人壁细胞中，并研究其与固有因子抗体和补体的细胞内结合，通过凝胶过滤将固有因子抗体与恶性贫血血清中共存的壁细胞抗体分离。通过半纯化的人内在因子– [ 57 Co] B12复合物免疫，兔中也产生了“结合”型和“阻断”型内在因子抗体。从这两种来源获得的内在因子抗体在存在荧光素的抗人IgG的间接Coons检验中，人壁细胞的荧光。荧光位于细胞膜的外围。当使用荧光抗人补体（C）血清和含正常补体的血清代替荧光抗人IgG时，两种来源的内源性因子抗体均可产生人粘膜整个壁细胞质的荧光。以最高浓度定位在人的壁细胞膜中，内源因子抗体-内在因子复合物在人壁细胞中被证实，并获得了这种抗原-抗体复合物固定补体的证据（C）。已经考虑到内源性因子-内源性因子抗体-补体复合物在恶性贫血的胃萎缩发展中的可能作用。

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