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首页> 美国卫生研究院文献>Clinical and Experimental Immunology >False positive ELISA tests for anticardiolipin antibodies in sera from patients with repeated abortion rheumatologic disorders and primary biliary cirrhosis: correlation with elevated polyclonal IgM and implications for patients with repeated abortion.
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False positive ELISA tests for anticardiolipin antibodies in sera from patients with repeated abortion rheumatologic disorders and primary biliary cirrhosis: correlation with elevated polyclonal IgM and implications for patients with repeated abortion.

机译：反复流产风湿性疾病和原发性胆汁性肝硬化患者血清抗心磷脂抗体的假阳性ELISA测试：与多克隆IgM升高的相关性以及对反复流产患者的影响。

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Non-specific binding in an ELISA test for antibodies to cardiolipin (ACA) was investigated in sera from patients with primary biliary cirrhosis, rheumatological disorders, and repeated abortion. Binding to wells without phospholipid was most frequent in ELISA assay for IgM class ACA and correlated with levels of serum polyclonal IgM in patients with repeated abortion and rheumatologic disorders (r = 0.79, 0.76). Using added polyclonal IgM we demonstrated that the increase in non-specific binding was most significant when levels of ACA were low; subtraction of binding to the unoccupied well over-corrected specific antibody estimates when a high affinity antibody was present. Absorption studies on three sera suggest that this 'non-specific' binding includes low affinity antibody binding. Elevated levels of polyclonal IgM in sera from patients with repeated abortion may account for some reports of elevated IgM class antibodies in other ELISA assays. The prevalence of a positive test for ACA in a sequential series of 412 such patients was reduced to 6.1% (95% confidence limits 4-9%) after subtraction of non-specific binding.
机译：在患有原发性胆汁性肝硬化，风湿病和反复流产的患者的血清中，研究了针对心磷脂（ACA）抗体的ELISA测试中的非特异性结合。对于反复流产和风湿性疾病的患者，在IgM AAC类ELISA检测中，结合最不含磷脂的孔最为常见，并且与血清多克隆IgM水平相关（r = 0.79，0.76）。使用添加的多克隆IgM，我们证明了，当ACA水平较低时，非特异性结合的增加最为明显。当存在高亲和力抗体时，减去与未被占用的，过度校正的特异性抗体的结合力。对三种血清的吸收研究表明，这种“非特异性”结合包括低亲和力抗体结合。反复流产患者血清中多克隆IgM的水平升高可能解释了其他ELISA分析中IgM类抗体水平升高的一些报道。扣除非特异性结合后，在412例此类患者中，ACA阳性检测的患病率连续降低至6.1％（95％置信度为4-9％）。

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