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The mechanisms of inhibitory effects of liver extract on lymphocyte proliferation: II. Inhibition of DNA RNA and protein synthesis and their relationship to the effects of metabolic inhibitors.

机译:肝提取物抑制淋巴细胞增殖的机制:II。 DNARNA和蛋白质合成的抑制作用及其与代谢抑制剂作用的关系。

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摘要

The inhibitory effects of liver extract (LEx) on DNA, RNA, and protein synthesis in phytohaemagglutinin (PHA)-stimulated lymphocytes were studied by measuring the incorporation of labelled precursors. DNA, RNA, and protein synthesis were all inhibited by LEx in a dose-related manner. The inhibitory effect of LEx on protein synthesis was additive to the inhibitory effect of puromycin. The inhibitory effect of LEx on DNA synthesis was antagonistic to the inhibitory effect of mitomycin C. In the case of actinomycin D for RNA synthesis, its interactions with LEx were variable, but not additive. LEx may act as an inhibitor of protein synthesis through the mechanism of arginine-depletion.
机译:通过测量标记前体的掺入,研究了肝提取物(LEx)对植物血凝素(PHA)刺激的淋巴细胞中DNA,RNA和蛋白质合成的抑制作用。 DNA,RNA和蛋白质的合成均受剂量相关的LEx抑制。 LEx对蛋白质合成的抑制作用与嘌呤霉素的抑制作用相加。 LEx对DNA合成的抑制作用与丝裂霉素C的抑制作用相反。在放线菌素D用于RNA合成的情况下,其与LEx的相互作用是可变的,但不是累加的。 LEx可以通过精氨酸消耗机制来抑制蛋白质的合成。

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