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美国卫生研究院文献>Journal of Tissue Engineering
>Microarchitectural analysis of decellularised unscarred and scarred dermis provides insight into the organisation and ultrastructure of the human skin with implications for future dermal substitute scaffold design
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Microarchitectural analysis of decellularised unscarred and scarred dermis provides insight into the organisation and ultrastructure of the human skin with implications for future dermal substitute scaffold design
The three-dimensional spatial arrangement of dermal tissue plays a crucial role in directing cellular behaviour during wound healing. It is vital to elucidate a better understanding of the three-dimensional dermal architecture of human skin. We sought to understand the configuration in morphological structure of decellularised human dermis between unscarred skin and normotrophic scars. Skin biopsies underwent decellularisation (DNA removal = 88%). Histological analysis showed no change in gross morphology of decellularised unscarred and scarred dermis. Multiphoton and atomic force microscopies showed that collagen fibres in unscarred decellularised dermis were interwoven akin to a mesh-like structure. Collagen fibres in decellularised unscarred dermis were less stiff (mean: 2.155 ± 0.9595 MPa; p < 0.0001) with a rougher (Rq = 16.5, Ra = 12.5, Rmax = 198; p < 0.0001) surface topography. Scarred dermis had a higher collagen volume density (papillary dermis, p < 0.0082; reticular dermis, p < 0.0332). The results demonstrate that scaffolds should exhibit a mesh-like structure with a biomimetic surface and low stiffness.
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机译：皮肤组织的三维空间排列在伤口愈合过程中指导细胞行为方面起着至关重要的作用。阐明对人类皮肤的三维真皮结构的更好理解至关重要。我们试图了解无疤痕皮肤和正常营养性瘢痕之间脱细胞人真皮形态结构的构型。皮肤活检进行脱细胞（脱DNA = 88％）。组织学分析显示，去细胞的无疤痕和疤痕真皮的总体形态没有变化。多光子和原子力显微镜检查表明，无疤的脱细胞真皮中的胶原纤维交织成网状结构。去细胞的无疤痕真皮中的胶原纤维的硬度较低（平均值：2.155±0.9595MPa； p <0.0001），表面形貌较粗糙（Rq = 16.5，Ra = 12.5，Rmax = 198； p <0.0001）。结疤的真皮具有更高的胶原蛋白体积密度（乳头状真皮，p 0.0082；网状真皮，p 0.0332）。结果表明，支架应表现出具有仿生表面和低硬度的网状结构。
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