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Effects of Full-Length Kisspeptin Administration on FollicularDevelopment in Japanese Black Beef Cows

机译:全长吻肽酶给药对卵泡的影响日本黑牛的发展

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摘要

Kisspeptin is a key molecule that stimulates gonadotropin secretion via release of gonadotropin-releasing hormone (GnRH). In the present study, our aim was to investigate whether kisspeptin has stimulatory effects on follicular development via GnRH/gonadotropin secretion in cows. Japanese Black beef cows were intravenously injected with full-length bovine kisspeptin [Kp-53 (0.2 or 2 nmol/kg)] or vehicle 5 days after they exhibited standing estrus (Day 0). In cows injected with Kp-53 at 2 nmol/kg, the follicular sizes of the first dominant follicles increased on Day 6 and thereafter. Ovulation of the first dominant follicle occurred in 1 out of 4 cows treated with Kp-53 at 2 nmol/kg. Injection of Kp-53 at 2 nmol/kg increased the concentration of plasma luteinizing hormone (LH) but not follicle-stimulating hormone, over a 4-h period following injection in all cows. The present study suggests that administration of full-length kisspeptin causes LH secretion, which is sustained for a few hours, and it is capable of stimulating follicular development and/or ovulation.
机译:Kisspeptin是通过释放促性腺激素释放激素(GnRH)刺激促性腺激素分泌的关键分子。在本研究中,我们的目的是研究牛乳中基瑟肽是否通过GnRH /促性腺激素分泌对卵泡发育具有刺激作用。在日本黑牛肉牛表现出发情状态后(第0天)静脉注射全长牛吻肽素[Kp-53(0.2或2 nmol / kg)]或媒介物。在以2 nmol / kg的剂量注射Kp-53的母牛中,第6天及其后第一个主要卵泡的卵泡大小增加。在以2 nmol / kg剂量的Kp-53处理的4头母牛中,有1头发生了第一卵泡的排卵。在所有母牛注射后的4小时内,以2 nmol / kg的剂量注射Kp-53会增加血浆促黄体生成激素(LH)的浓度,但不会增加促卵泡激素的浓度。本研究表明全长kisepteptin的给药会导致LH分泌,持续持续数小时,并且能够刺激卵泡发育和/或排卵。

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