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首页> 美国卫生研究院文献>The Journal of Reproduction and Development >Comparison of Cryotop and micro volume air cooling methods for cryopreservation of bovine matured oocytes and blastocysts
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Comparison of Cryotop and micro volume air cooling methods for cryopreservation of bovine matured oocytes and blastocysts

机译：冷冻保存的牛成熟卵母细胞和囊胚的冷冻和微量风冷方法的比较

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摘要

This study was designed to compare the efficiency of the Cryotop method and that of two methods that employ a micro volume air cooling (MVAC) device by analyzing the survival and development of bovine oocytes and blastocysts vitrified using each method. In experiment I, in vitro-matured (IVM) oocytes were vitrified using an MVAC device without direct contact with liquid nitrogen (LN2; MVAC group) or directly plunged into LN2 (MVAC in LN2 group). A third group of IVM oocytes was vitrified using a Cryotop device (Cryotop group). After warming, vitrified oocytes were fertilized in vitro. There were no significant differences in cleavage and blastocyst formation rates among the three vitrified groups, with the rates ranging from 53.1% to 56.6% and 20.0% to 25.5%, respectively; however, the rates were significantly lower (P < 0.05) than those of the fresh control group (89.3% and 43.3%, respectively) and the solution control group (87.3% and 42.0%, respectively). In experiment II, in vitro-produced (IVP) expanded blastocysts were vitrified using the MVAC, MVAC in LN2 and Cryotop methods, warmed and cultured for survival analysis and then compared with the solution control group. The rate of development of vitrified-warmed expanded blastocysts to the hatched blastocyst stage after 24 h of culture was lower in the MVAC in LN2 group than in the solution control group; however, after 48–72 h of culture, the rates did not significantly differ between the groups. These results indicate that the MVAC method without direct LN2 contact is as effective as the standard Cryotop method for vitrification of bovine IVM oocytes and IVP expanded blastocysts.
机译：本研究旨在通过分析每种方法玻璃化的牛卵母细胞和胚泡的存活和发育情况，来比较Cryotop方法和两种采用微风冷（MVAC）设备的方法的效率。在实验I中，使用MVAC装置将体外成熟（IVM）卵母细胞玻璃化，而不直接与液氮接触（LN2； MVAC组），或直接插入LN2（LN2组中的MVAC）。使用Cryotop装置将第三组IVM卵母细胞玻璃化（Cryotop组）。温热后，将玻璃化的卵母细胞体外受精。三个玻璃化组之间的卵裂和囊胚形成率没有显着差异，分别为53.1％至56.6％和20.0％至25.5％。但是，该比例显着低于新鲜对照组（分别为89.3％和43.3％）和溶液对照组（分别为87.3％和42.0％）（P <0.05）。在实验II中，使用MVAC，LN2中的MVAC和Cryotop方法将体外产生的（IVP）膨胀胚泡玻璃化，加热并培养以进行生存分析，然后与溶液对照组进行比较。 LN2组的MVAC组在培养24小时后，玻璃化温热的膨胀胚泡发育到孵化的胚泡期的速率低于溶液对照组。然而，在培养48-72小时后，两组之间的比率没有显着差异。这些结果表明，不直接与LN2接触的MVAC方法与用于牛IVM卵母细胞和IVP扩增的胚泡玻璃化的标准Cryotop方法一样有效。

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