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首页> 美国卫生研究院文献>Breeding Science >Production and characterization of an alloplasmic and monosomic addition line of Brassica rapa carrying the cytoplasm and one chromosome of Moricandia arvensis
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Production and characterization of an alloplasmic and monosomic addition line of Brassica rapa carrying the cytoplasm and one chromosome of Moricandia arvensis

机译：带有胞质和一条桑of的一条芸苔的同质异位加成系的生产和鉴定

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Intergeneric hybridization was performed between Moricandia arvensis and four inbred lines of Brassica rapa following embryo rescue. Three F1 hybrid plants were developed from three cross combinations of M. arvensis × B. rapa, and amphidiploids were synthesized by colchicine treatment. Six BC1 plants were generated from a single cross combination of amphidipolid × B. rapa ‘Ko1-303’ through embryo rescue. One BC2 and three BC3 plants were obtained from successive backcrossing with B. rapa ‘Ko1-303’ employing embryo rescue. Alloplasmic and monosomic addition lines of B. rapa (Allo-MALs, 2n = 21) were obtained from backcrossed progeny of three BC3 plants (2n = 21, 22 and 23) without embryo rescue. An alloplasmic line of B. rapa (2n = 20) degenerated before floliation on 1/2 MS medium due to severe chlorosis. Allo-MALs of B. rapa (2n = 21) showed stable male sterility without any abnormal traits in vegetative growth and female fertility. Molecular analyses revealed that the same chromosome and cytoplasm of M. arvensis had been added to each Allo-MAL of B. rapa. This Allo-MAL of B. rapa may be useful material for producing cytoplasmic male sterile lines of B. rapa.
机译：胚胎抢救后，Moricandia arvensis和四个甘蓝型自交系之间进行属间杂交。由阿尔维螺旋体×野菜的三种交叉组合培育出3株F1杂种植株，秋水仙碱处理合成了二倍体。六种BC1植株是通过两性交代的两性生殖×ra。rapa'Ko1-303'通过胚胎拯救而产生的。通过使用胚胎抢救与连续芽孢杆菌“ Ko1-303”进行连续回交，获得了1株BC2和3株BC3植物。从三株BC3植物（2n = 21、22和23）的回交后代中获得了B. rapa的异质和单体添加系（Allo-MALs，2n = 21），而没有胚胎拯救。由于严重的萎黄病，在1/2 MS培养基上形成叶之前，B。rapa（2n = 20）的同质系退化。 B. rapa（2n = 21）的Allo-MALs表现出稳定的雄性不育，在营养生长和雌性育性上没有任何异常特征。分子分析表明，相同的染色体和胞质分枝杆菌已被添加到B. rapa的每个异源MAL中。 B. rapa的Allo-MAL可能是生产B. rapa的胞质雄性不育系的有用材料。

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