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Fine mapping of the major Soybean dwarf virus resistance gene Rsdv1 of the soybean cultivar ‘Wilis’

机译:大豆品种 Wilis的主要大豆矮化病毒抗性基因Rsdv1的精细定位

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摘要

Soybean dwarf virus (SbDV), a Luteoviridae family member, causes dwarfing, yellowing and sterility of soybean (Glycine max), leading to one of the most serious problems in soybean production in northern Japan. Previous studies revealed that the Indonesian soybean cultivar ‘Wilis’ is resistant to SbDV and that the resistance can be introduced into Japanese cultivars. A major QTL for SbDV resistance has been reported between SSR markers Sat_217 and Satt211 on chromosome 5. In this study, we named this QTL Rsdv1 (resistance to SbDV) and developed near-isogenic lines incorporating Rsdv1 (Rsdv1-NILs) using Sat_217 and Satt211 markers. The Rsdv1-NILs were resistant to SbDV in greenhouse inoculation and field tests, indicating that Rsdv1 alone is sufficient for the resistance phenotype. We fine-mapped Rsdv1 within the 44-kb region between Sat_11 and Sct_13. None of the six genes predicted in this region was closely related to known virus resistance genes in plants. Thus, Rsdv1 may confer resistance by a previously unknown mechanism. We suggest that Rsdv1 may be a useful source for the Japanese soybean breeding program to introduce SbDV resistance.
机译:黄体病毒科成员大豆矮病毒(SbDV)引起大豆(Glycine max)的矮化,发黄和不育,导致日本北部大豆生产中最严重的问题之一。先前的研究表明,印尼大豆品种“ Wilis”对SbDV具有抗性,并且该抗性可以引入日本品种。据报道,第5号染色体上的SSR标记Sat_217和Satt211之间存在主要的SbDV抗性QTL。在这项研究中,我们将其命名为QTL Rsdv1(对SbDV的抗性),并使用Sat_217和Satt211开发了包含Rsdv1(Rsdv1-NILs)的近等基因系。标记。在温室接种和田间试验中,Rsdv1-NIL对SbDV具有抗性,表明仅Rsdv1足以满足抗性表型。我们在Sat_11和Sct_13之间的44 KB区域内精细映射了Rsdv1。该区域预测的六个基因中没有一个与植物中已知的病毒抗性基因密切相关。因此,Rsdv1可以通过以前未知的机制赋予抗性。我们建议Rsdv1可能是日本大豆育种计划引入SbDV抗性的有用来源。

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