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Mapping of a major QTL associated with protein content on chromosome 2B in hard red winter wheat (Triticum aestivum L.)

机译:硬红冬小麦(Triticum aestivum L.)中与2B染色体上蛋白质含量相关的主要QTL定位

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摘要

A quantitative trait locus (QTL) controlling wheat grain protein content (GPC) and flour protein content (FPC) was identified using doubled haploid (DH) lines developed from a cross between the hard red winter wheat variety ‘Yumechikara’ with a high protein content used for bread making, and the soft red winter wheat ‘Kitahonami’ with a low protein content used for Japanese white salted noodles. A single major QTL, QGpc.2B-yume, was identified on the short arm of wheat chromosome 2>B for both the GPC and FPC over 3 years of testing. QGpc.2B-yume was mapped on the flanking region of microsatellite marker Xgpw4382. The DH lines grouped by the haplotype of the closest flanking microsatellite marker Xgpw4382 showed differences of 1.0% and 1.1% in mean GPC and FPC, respectively. Yield-component-related traits were not affected by the haplotype of QGpc.2B-yume, and major North American hard red winter wheat varieties showed the high-protein haplotype. Unlike Gpc-B1 derived from tetraploid wheat, QGpc.2B-yume has no negative effects on yield-component-related traits and should be useful for wheat breeding to increase GPC and FPC.
机译:使用从高蛋白质含量的硬红冬小麦品种“ Yumechikara”之间杂交得到的双倍单倍体(DH)系,确定了控制小麦籽粒蛋白质含量(GPC)和面粉蛋白质含量(FPC)的数量性状位点(QTL)用于面包制作,以及柔软的红色冬小麦'Kitahonami',其蛋白质含量低,用于日本白盐面条。经过三年的测试,在GPC和FPC的小麦2号染色体> B 的短臂上鉴定出一个主要的QTL QGpc.2B-yume。 QGpc.2B-yume被定位在微卫星标记Xgpw4382的侧翼区域。由最接近的侧翼微卫星标记Xgpw4382的单倍型分组的DH品系的平均GPC和FPC差异分别为1.0%和1.1%。产量相关的性状不受QGpc.2B-yume单倍型的影响,北美主要的硬红冬小麦品种表现出高蛋白单倍型。与源自四倍体小麦的Gpc-B1不同,QGpc.2B yume对与产量构成相关的性状没有负面影响,应该对小麦育种有用以增加GPC和FPC。

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