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A practical rapid generation-advancement system for rice breeding using simplified biotron breeding system

机译:一种实用的利用简化的生物加速育种系统快速进行水稻育种的先进系统

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摘要

A new plant breeding method—the biotron breeding system (BBS)—can rapidly produce advanced generations in rice (Oryza sativa L.) breeding. This method uses a growth chamber (biotron) with CO2 control, accompanied by tiller removal and embryo rescue to decrease the period before seed maturity. However, tiller removal and embryo rescue are laborious and impractical for large populations. We investigated the influences of increased CO2, tiller removal, and root restriction on the days to heading (DTH) from seeding in growth chambers. The higher CO2 concentration significantly decreased DTH, but tiller removal and root restriction had little effect on DTH and drastically reduced seed yield. Based on these findings, we propose a simplified BBS (the sBBS) that eliminates the need for tiller removal and embryo rescue, but controls CO2 levels and day-length and maintains an appropriate root volume. Using the sBBS, we could reduce the interval between generations in ‘Nipponbare’ to less than 3 months, without onerous manipulations. To demonstrate the feasibility of the sBBS, we used it to develop isogenic lines using ‘Oborozuki’ as the donor parent for the low-amylose allele Wx1-1 and ‘Akidawara’ as the recipient. We were able to perform four crossing cycles in a year.
机译:一种新的植物育种方法-生物增子育种系统(BBS)-可以在水稻(Oryza sativa L.)育种中快速产生先进的后代。该方法使用带有CO2控制的生长室(biotron),同时进行分till除去和胚胎挽救,以缩短种子成熟之前的时间。然而,对于大量人群而言,去除分removal和挽救胚胎是费力且不切实际的。我们调查了CO2增加,分till去除和根系限制对从生长室播种到抽穗的天数(DTH)的影响。较高的CO2浓度显着降低了DTH,但分till去除和根系限制对DTH的影响很小,并且大大降低了种子产量。基于这些发现,我们提出了一种简化的BBS(sBBS),它消除了分till清除和胚胎抢救的需要,但可以控制CO2水平和日长,并保持适当的根部体积。使用sBBS,我们可以将“日本晴”中的世代间隔缩短到不到3个月,而无需进行繁琐的操作。为了证明sBBS的可行性,我们使用它来开发等基因系,使用'Oborozuki'作为低直链淀粉等位基因Wx1-1的供体亲本和'Akidawara'作为受体。我们一年可以执行四个穿越周期。

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