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首页> 美国卫生研究院文献>Breeding Science >Screening of clubroot-resistant varieties and transfer of clubroot resistance genes to Brassica napus using distant hybridization
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Screening of clubroot-resistant varieties and transfer of clubroot resistance genes to Brassica napus using distant hybridization

机译：远缘杂交筛选抗根瘤病品种并将抗根病基因转移至甘蓝型油菜

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摘要

Clubroot is an economically important disease affecting plants in the family Cruciferae worldwide. In this study, a collection of 50 Cruciferae accessions was screened using Plasmodiophora brassicae pathotype 4 in China. Eight of these demonstrated resistance, including three Chinese cabbages, two cabbages, one radish, one kale, and one Brassica juncea. The three clubroot-resistant Chinese cabbages (1003, 1007 and 1008) were then used to transfer the clubroot resistance genes to B. napus by distant hybridization combined with embryo rescue. Three methods including morphological identification, cytology identification, and molecular marker-assisted selection were used to determine hybrid authenticity, and 0, 2, and 4 false hybrids were identified by these three methods, respectively. In total, 297 true hybrids were identified. Clubroot resistance markers and artificial inoculation were utilized to determine the source of clubroot resistance in the true hybrids. As a result, two simple sequence repeat (SSR) and two intron polymorphic (IP) markers linked to clubroot resistance genes were identified, the clubroot resistance genes of 1007 and 1008 were mapped to A03. At last, 159 clubroot-resistant hybrids were obtained by clubroot resistance markers and artificial inoculation. These intermediate varieties will be used as the ‘bridge material’ of clubroot resistance for further B. napus breeding.
机译：Clubroot是一种经济重要的疾病，会影响全世界十字花科的植物。在这项研究中，使用中国的芸苔疟原虫病原体4筛选了50个十字花科种质。其中有八株表现出抗性，包括三株大白菜，两株白菜，一根萝卜，一根羽衣甘蓝和一株芥菜。然后，通过远距离杂交结合胚胎抢救，使用三种抗根癌的大白菜（1003、1007和1008）将抗根癌的基因转移至甘蓝型油菜。形态学鉴定，细胞学鉴定和分子标记辅助选择三种方法用于确定杂种的真实性，这三种方法分别鉴定出0、2和4个假杂种。总共鉴定出297个真正的杂种。根瘤病抗性标记和人工接种被用来确定真正杂种中根瘤病抗性的来源。结果，鉴定了与根部抗性基因连锁的两个简单序列重复（SSR）和两个内含子多态性（IP）标记，将根部抗性基因1007和1008定位到A03。最后，通过抗根茎标记和人工接种获得了159个抗根茎的杂种。这些中间品种将用作抗根癌的“桥梁材料”，以进一步促进甘蓝型油菜的繁殖。

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