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Effect of acid hydrolysis and fungal biotreatment on agro-industrial wastes for obtainment of free sugars for bioethanol production

机译:酸水解和真菌生物处理对农业工业废料获得游离糖以生产生物乙醇的影响

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摘要

This study was designed to evaluate selected chemical and microbiological treatments for the conversion of certain local agro-industrial wastes (rice straw, corn stalks, sawdust, sugar beet waste and sugarcane bagasse) to ethanol. The chemical composition of these feedstocks was determined. Conversion of wastes to free sugars by acid hydrolysis varied from one treatment to another. In single-stage dilute acid hydrolysis, increasing acid concentration from 1 % (v/v) to 5 % (v/v) decreased the conversion percentage of almost all treated agro-industrial wastes. Lower conversion percentages for some treatments were obtained when increasing the residence time from 90 to 120 min. The two-stage dilute acid hydrolysis by phosphoric acid (1.0 % v/v) followed by sulphuric acid (1.0 % v/v) resulted in the highest conversion percentage (41.3 % w/w) on treated sugar beet waste. This treatment when neutralized, amended with some nutrients and inoculated with baker’s yeast, achieved the highest ethanol concentration (1.0 % v/v). Formation of furfural and hydroxymethylfurfural (HMF) were functions of type of acid hydrolysis, acid concentration, residence time and feedstock type. The highest bioconversion of 5 % wastes (37.8 % w/w) was recorded on sugar beet waste by Trichoderma viride EMCC 107. This treatment when followed by baker’s yeast fermentation, 0.41 % (v/v) ethanol and 8.2 % (v/w) conversion coefficient were obtained.
机译:这项研究旨在评估某些局部农业工业废物(稻草,玉米秸秆,锯末,甜菜废料和甘蔗渣)向乙醇转化的选定化学和微生物处理方法。确定了这些原料的化学组成。通过酸水解将废物转化为游离糖的方法从一种处理到另一种处理有所不同。在单级稀酸水解中,酸浓度从1%(v / v)增加到5%(v / v),会降低几乎所有处理过的农业工业废料的转化率。当停留时间从90分钟增加到120分钟时,某些处理的转化率较低。用磷酸(1.0%v / v)然后用硫酸(1.0%v / v)进行两阶段的稀酸水解,在处理过的甜菜废料上转化率最高(41.3%w / w)。中和,添加了一些营养素并接种了面包酵母后,这种处理达到了最高的乙醇浓度(1.0%v / v)。糠醛和羟甲基糠醛(HMF)的形成是酸水解类型,酸浓度,停留时间和原料类型的函数。通过甜菜木霉EMCC 107在甜菜废物上记录了最高的5%废物生物转化(37.8%w / w)。在面包师酵母发酵,0.41%(v / v)乙醇和8.2%(v / w)之后进行此处理获得了转换系数。

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