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首页> 美国卫生研究院文献>Brazilian Journal of Microbiology >Optimization of liquid fermentation conditions for biotransformation zein by Cordyceps militaris 202 and characterization of physicochemical and functional properties of fermentative hydrolysates
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Optimization of liquid fermentation conditions for biotransformation zein by Cordyceps militaris 202 and characterization of physicochemical and functional properties of fermentative hydrolysates

机译：Cord虫草202对生物转化玉米蛋白的液体发酵条件的优化及发酵水解物的理化和功能性质表征

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Cordyceps militaris 202 is a potential fungus for biotransformation zein, due to its various proteases, high tolerance and viability in nature. In this article, single factor experiment and response surface methodology were applied to optimize the liquid fermentation conditions and improve the ability of biotransformation zein. The optimized fermentation conditions were as follows: inoculum concentration of 19%, volume of liquor of 130 mL/500 mL and pH of 4.7. Under this condition, the degree of hydrolysis (DH) was 27.31%. The zein hydrolysates from fungi fermentation maintained a high thermal stability. Compared to the original zein, the zein hydrolysates were found to have high solubility, which most likely results in improved foaming and emulsifying properties. Overall, this research demonstrates that hydrolysis of zein by C. militaris 202 is a potential method for improving the functional properties of zein, and the zein hydrolysates can be used as functional ingredients with an increased antioxidant effect in both food and non-food applications.
机译：虫草202由于其多种蛋白酶，天然的高耐受性和生存力，是生物转化玉米醇溶蛋白的潜在真菌。本文采用单因素实验和响应面方法优化了液体发酵条件，提高了玉米醇溶蛋白的生物转化能力。优化的发酵条件如下：接种物浓度为19％，液体体积为130mL / 500mL，pH为4.7。在此条件下，水解度（DH）为27.31％。来自真菌发酵的玉米醇溶蛋白水解产物保持高的热稳定性。与原始玉米醇溶蛋白相比，发现玉米醇溶蛋白水解产物具有高溶解度，这最有可能导致改善的起泡和乳化性能。总体而言，这项研究表明，米克氏菌202水解玉米醇溶蛋白是改善玉米醇溶蛋白功能特性的一种潜在方法，并且玉米醇溶蛋白水解产物可以用作功能性成分，在食品和非食品应用中均具有增强的抗氧化作用。

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