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首页> 美国卫生研究院文献>The Journal of Physiology >Noradrenaline modulates calcium channels in avian dorsal root ganglion cells through tight receptor-channel coupling.
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Noradrenaline modulates calcium channels in avian dorsal root ganglion cells through tight receptor-channel coupling.

机译：去甲肾上腺素通过紧密的受体-通道偶联调节禽背根神经节细胞中的钙通道。

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摘要

Averaged ensemble Ba currents were recorded from tissue cultured embryonic chick dorsal root ganglion (d.r.g.) cells using the cell-attached patch-clamp technique. Noradrenaline (NA) applied to extrapatch membrane had no clear consistent effect on drug-free patch currents. This finding supports a previous suggestion that second messengers may not be involved in NA-mediated decreases in Ca currents in sensory neurones (Forscher & Oxford, 1985). Cell-attached patch currents sometimes increased slowly after extrapatch application of NA, but were not reversibly decreased by drug treatment. Large patch currents were used to trigger cellular action potentials. NA reversibly decreased action potential duration as reflected in extracellularly recorded patch action currents. Simultaneously recorded inward patch currents were not affected. D.r.g. cell adenylate cyclase activity was assayed. NA did not affect intracellular cyclic AMP levels at concentrations which cause 30-70% decreases in gCa in dialysed cells (Forscher & Oxford, 1985). Treatment with forskolin (50 microM) or isoprenaline (10 microM) resulted in 60- and 2-fold increases respectively in adenylate cyclase activity over basal levels. These results suggest that NA decreases Ca currents by direct NA interactions with the Ca channel or a molecule tightly coupled to channel function in d.r.g. cells.
机译：使用贴附细胞的膜片钳技术从组织培养的胚胎鸡背根神经节（d.r.g.）细胞中记录平均集合Ba电流。去甲膜上使用去甲肾上腺素（NA）对无药贴剂电流没有明显的一致影响。这一发现支持了以前的观点，即第二信使可能不参与NA介导的感觉神经元Ca电流的降低（Forscher＆Oxford，1985）。细胞外贴片电流有时在膜外应用NA后缓慢增加，但并未通过药物治疗可逆地降低。使用大的贴片电流来触发细胞动作电位。 NA可逆地减少了动作电位的持续时间，这反映在细胞外记录的贴片动作电流中。同时记录的内向贴片电流不受影响。 D.r.g.测定细胞腺苷酸环化酶活性。在引起透析细胞的gCa降低30-70％的浓度下，NA不会影响细胞内循环AMP的水平（Forscher＆Oxford，1985）。用福司可林（50 microM）或异戊二烯（10 microM）处理导致腺苷酸环化酶活性分别比基础水平高60倍和2倍。这些结果表明，NA通过与钙通道或紧密结合于d.r.g中的通道功能的分子的直接NA相互作用而降低了钙电流。细胞。

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