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首页> 美国卫生研究院文献>British Journal of Cancer >In vitro drug sensitivity of normal peripheral blood lymphocytes and childhood leukaemic cells from bone marrow and peripheral blood.
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In vitro drug sensitivity of normal peripheral blood lymphocytes and childhood leukaemic cells from bone marrow and peripheral blood.

机译：正常外周血淋巴细胞和来自骨髓和外周血的儿童白血病细胞的体外药物敏感性。

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In vitro drug sensitivity of leukaemic cells might be influenced by the contamination of such a sample with non-malignant cells and the sample source. To study this, sensitivity of normal peripheral blood (PB) lymphocytes to a number of cytostatic drugs was assessed with the MTT assay. We compared this sensitivity with the drug sensitivity of leukaemic cells of 38 children with acute lymphoblastic leukaemia. We also studied a possible differential sensitivity of leukaemic cells from bone marrow (BM) and PB. The following drugs were used: Prednisolone, dexamethasone, 6-mercaptopurine, 6-thioguanine, cytosine arabinoside, vincristine, vindesine, daunorubicin, doxorubicin, mafosfamide (Maf), 4-hydroperoxy-ifosfamide, teniposide, mitoxantrone, L-asparaginase, methotrexate and mustine. Normal PB lymphocytes were significantly more resistant to all drugs tested, except to Maf. Leukaemic BM and PB cells from 38 patients (unpaired samples) showed no significant differences in sensitivity to any of the drugs. Moreover, in 11 of 12 children with acute leukaemia of whom we investigated simultaneously obtained BM and PB (paired samples), their leukaemic BM and PB cells showed comparable drug sensitivity profiles. In one patient the BM cells were more sensitive to most drugs than those from the PB, but the actual differences in sensitivity were small. We conclude that the contamination of a leukaemic sample with normal PB lymphocytes will influence the results of the MTT assay. The source of the leukaemic sample, BM or PB, does not significantly influence the assay results.
机译：白血病细胞的体外药物敏感性可能受到此类样品被非恶性细胞和样品来源污染的影响。为了研究这一点，使用MTT分析法评估了正常外周血（PB）淋巴细胞对多种细胞抑制药物的敏感性。我们将这种敏感性与38例急性淋巴细胞白血病儿童的白血病细胞的药物敏感性进行了比较。我们还研究了来自骨髓（BM）和PB的白血病细胞的可能差异敏感性。使用了以下药物：泼尼松龙，地塞米松，6-巯基嘌呤，6-硫鸟嘌呤，阿糖胞苷，长春新碱，长春地辛，柔红霉素，阿霉素，马夫草胺（Maf），4-氢过氧-异环磷酰胺，替尼泊苷，米托蒽醌，甲氨蝶呤，甲氨蝶呤，甲氨蝶呤芥末酱。正常PB淋巴细胞对所有测试的药物（除黑手党以外）的抵抗力均明显更高。 38名患者（未配对样品）的白血病BM和PB细胞对任何药物的敏感性均无显着差异。此外，在我们调查同时获得的BM和PB（配对样本）的12例急性白血病儿童中，他们的白血病BM和PB细胞显示出可比的药物敏感性谱。一名患者的BM细胞对大多数药物的敏感性高于PB细胞，但敏感性的实际差异很小。我们得出的结论是，正常PB淋巴细胞对白血病样本的污染将影响MTT分析的结果。白血病样品的来源BM或PB不会显着影响测定结果。

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